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大鼠甲状旁腺细胞体外培养及功能的实验研究 被引量:3

Experimental study on the cell culture and secretory function of parathyroid cells in rats
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摘要 目的:探讨大鼠甲状旁腺细胞的培养方法及其分泌功能,为甲状旁腺细胞移植奠定实验基础。方法:胶原酶和胰蛋白酶系列消化甲状旁腺后,进行细胞培养。透射电镜检查培养5d的甲状旁腺细胞,对1、3、5、7d的细胞培养液行甲状旁腺激素测定。结果:培养5d的甲状旁腺细胞具有丰富的粗面内质网、高尔基复合体、杆状的线粒体和分泌颗粒。甲状旁腺细胞经过培养后可以正常分泌甲状旁腺激素,以第5天为最佳。结论:培养5d的甲状旁腺细胞仍保持原有的形态结构,分泌功能最强,可作为同种移植的最佳供体。 Objective:To investigate the culture technique and secretory function of parathyroid cells in rats in order to establish experimental basis of parathyroid cell transplantation.Methods:After parathyroid glands were digested by collagenase and trypsin,isolated parathyroid cells were cultured.The cells cultured for 5 days were observed by transmission electron microscopy.Parathyroid hormone obtained from the cell culture fluid on day 1,3,5 and 7 was measured respectively.Results:Parathyroid cells cultured for 5 days had abundant rough surfaced endoplasmic reticula、Golgi bodies、stab mitochondria and secretory granules.Cultured parathyroid cells could secrete parathyroid hormone normally,especially on the 5th day.Conclusion:Parathyroid cells cultured for 5 days still possess original morphologic structure and have maximum secreting function,so it is an optimal donor for transplantation.
出处 《中国现代普通外科进展》 CAS 2008年第5期389-391,共3页 Chinese Journal of Current Advances in General Surgery
关键词 甲状旁腺 细胞培养 甲状旁腺激素 Parathyroid gland·Cell culture·Parathyroid hormone
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参考文献4

  • 1Marx SJ. Hyperparathyroid and hypoparathyroid disorders[J]. N Engl J Med, 2000,343(25):1863-1875.
  • 2Drueke TB, Cattan P, Zingraff J, et al. Endocrine-cell allotransplantation[J]. Lancet, 1998,351(9120): 597-598.
  • 3Tsuji K, Fuehinoue S, Kai K, et al. Culture of human parathyroid cells for transplantation[J]. Transplant Proc, 1999,31(7):2697.
  • 4Faustman D, Coe C. Prevention of xenograft rejection by masking donor HLA class 1 antigens[J]. Science, 1991,252(5013):1700-1702.

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