摘要
目的:研究抗凋亡基因Bag-1和Bcl-2在结肠癌组织中的表达及意义,并探讨二者之间的关系。方法:应用免疫组织化学SABC法(链霉亲合素-生物素-过氧化物酶复合物法)对128例结肠癌组织及20例正常结肠组织中Bag-1和Bcl-2基因的表达进行检测。结果:Bag-1和Bcl-2在结肠癌中的表达与结肠癌病理类型、肿瘤直径、浸润深度及有无淋巴结转移无关,但Bag-1与病理分级、远处转移、Dukes分期及预后密切相关(P<0.05),而Bcl-2与这几项临床病理因素无明显相关性(P>0.05);Bag-1和Bcl-2在结肠癌的发生发展过程中呈正相关(r=0.475)。结论:结肠癌组织中有不同水平的Bag-1和Bcl-2蛋白的高表达,两者相互作用并可以作为结肠癌早期筛选的一项指标,对疾病的预后有重要意义。
Objective：To investigate the expressions of Bag-1 and Bcl-2 in colorectal cancer and evaluate their clinical significance.Methods：Bag-1 and Bcl-2 expressions were studied by immunohistochemical Streptavidin-Biotin-Peroxidase Complex（SABC）method in 64 samples of colorectal cancer tissues,10 normal colorectal tissues.Results：Bag-1 was positively related to the tumor grade,distant metastasis,stages of Dukes and prognosis,but was not related to the pathological cell types,tumor diameter,depth of invasion and lymph node metastasis.Bcl-2 was not correlated to these clinicopathologic factors.There was a positive correlation between Bag-1 and Bcl-2（r=0.475）.Conclusion：The over expression of Bag-1 and Bcl-2 protein in colorectal cancer can affect the generation of colorectal cancer by participating in the regulation of apoptosis.
出处
《中国现代普通外科进展》
CAS
2008年第5期395-398,共4页
Chinese Journal of Current Advances in General Surgery
基金
国家863项目资助(2001AA218051)
