摘要
目的了解温度、时间、抗凝剂、不同试剂、不同仪器等因素对病毒性肝炎患者血清酶测定的影响。方法采集患者血清分别比较加抗凝剂、放置不同时间、不同温度保存24h、不同试剂、不同仪器对测定的影响。结果采用肝素锂抗凝影响腺苷脱氨酶ADA、总补体CH50的测定；室温放置24h（不分离血清）将影响ADA、CH50的测定；不同厂家试剂将影响结果的一致性。结论不同保存条件和试剂将影响测定结果。为了使检测结果有较好的一致性，必须制订和执行标准化的操作规程。
Objective To study some factors （temperature, time, anticoagulate, reagent, apparatus） which influence the detection of serum enzyme. Methods Serums obtained from same patient are determined in different conditions. Compare the statistical difference among each group. Results The anticoagulant-heparinize Li influence the determination of ADA and CH50. If serums were sent in room temperature for 24 hours, the results will be different. Using regents from different factories will receive different results. Conclusion Different conditions and reagents will influence results. To obtain correct data, we must institute and perform standard regulation.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2008年第5期385-387,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
血清酶
补体
腺苷脱氨酶
Serum enzyme
Complement
Adenosine deaminase
