摘要
文章介绍了一种不依赖于传统培养方法的限制性酶切片段长度多态性技术(RFLP),在湖泊微生物多样性研究中的应用、及其优缺点,并针对湖泊微生物基因组DNA的提取方法和16SrDNA片段的PCR优化条件等方面进行了探讨。
Principle and method of restriction fragment length polymorphism（RFLP） which was culture-independent were described, with its application in microbial diversity of lake as well as its advantages and disadvantages. The method of DNA extraction from lake microbes and the optimal conditions for PCR amplification of 16SrDNA were also discussed.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2008年第11期9-12,17,共5页
Environmental Science & Technology
基金
江西省自然科学基金资助项目(2007GZN1927)
同济大学长江水环境教育部重点实验室开放基金资助项目(YRWEF07005)
关键词
湖泊微生物
多样性
16SrDNA
RFLP
lake microbes
diversity
16SrDNA
restriction fragment length polymorphism（RFLP）
