摘要
[目的]提高优良绣球属花卉品种"尼克兰"的繁殖速度。[方法]选用"尼克兰"健壮幼嫩茎尖作为组培试验材料,进行组培快繁的研究。[结果]结果表明:诱导愈伤组织形成的培养基组合为MS+BA 2.0 mg/L+NAA 0.1 mg/L,有利于愈伤组织生长;不定芽分化的培养基为MS+BA 2.0 mg/L+NAA 0.1 mg/L,有利于不定芽的分化;而4/5MS+BA 2.0 mg/L+NAA 0.1 mg/L有利于组培苗不定根的形成。[结论]在"尼克兰"的组培繁殖中,重要的是注意茎尖和环境的消毒。
[ Objective ] The study aimed to improve the breeding speed of Hydrangea macrophylla ＇ Nikko Blue＇. [ Method ] Vigorous and heahhy shoot tips of H. macrophylla ＇ Nikko Blue＇ was selected as explants, and used to carry out the plant tissue culture technology research. [ Result] The results showed that plant hormone combination of MS ＋ BA 2.0 mg/L ＋ NAA 0.1 mg/L was good for callus induction , MS ＋ BA 2.0 mg/L ＋ NAA 0.1 mg/L was suitable for adventitious bud formation ,4/5 MS ＋ BA 2.0 mgcZL ＋ NAA 0.1 mg/L was good for root formation. [ Conclusion] During the tissue culture of H. macrophylla ＇ Nikko Blue＇ ,the disinfection of shoot tip and environment was very important.
出处
《安徽农业科学》
CAS
北大核心
2008年第29期12581-12581,12809,共2页
Journal of Anhui Agricultural Sciences
关键词
绣球品种
组织培养
适应性
Hydrangea macrophylla ＇ Nikko Blue＇
Tissue culture
Adaptability
