摘要
[目的]建立洋桔梗高效的快速繁殖体系,为工厂化生产提供可行的操作程序。[方法]以国外进口的洋桔梗F1代种子(品种名称为Polestar yellow)无菌苗的叶片为外植体,对不定芽的诱导、单芽增殖、无菌苗生根等进行了研究。[结果]结果表明:6-BA和IBA组合对洋桔梗叶片不定芽的诱导效果较6-BA和NAA组合好,叶片最佳的分化培养基为MS+6-BA0.6 mg/L+IBA0.04 mg/L,1个月内正常芽平均分化数为8.3;最佳的单芽增殖培养基为MS+6-BA0.8 mg/L+NAA0.04 mg/L,1个月内正常芽的增殖倍数为7.4;无菌苗在含有IBA0.25 mg/L的1/2 MS培养基上,生根率、平均每苗的根数均最大。[结论]该结果为洋桔梗的工厂化生产奠定了基础。
[ Objective] This study aimed to establish a feasible manipulation procedure for rapid propagation of a new Eustoma grandiflorum species in a faetory. [ Method] Using the aseptic seedling leaves of a newly imported Eustoma grandiflorum species （Polestar yellow） as explants, induction of the adventitious buds, proliferation of single buds and root regeneration of seedlings were studied. [ Result ] The results showed that, for the induetion effect of the adventitious buds from leaves, the eombination of 6-BA and IBA was better than that of 6-BA and NAA. The best bud induction medium for Polestar yellow was MS ＋ 6-BA 0.6 mg/L ＋ IBA 0.04 mg/L, and the mean number of the normal induced buds was 8.3 per month. The best proliferation medium was MS ＋ 6-BA 0.8 mg/L ＋ NAA 0.04 ing/L, and the proliferation multi- ple of the normal buds was 7.4 per month. On 1/2 MS medium with IBA O. 25 mg/L, both the root regeneration rate and the mean root number of the plantlets were highest. [ Conclulsion] These results established a basis for factory production of the new Eustoma grandiflorum species, Polestar yellow
出处
《安徽农业科学》
CAS
北大核心
2008年第29期12582-12584,12586,共4页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30670163)
教育部留学回国人员科研启动基金(2005-55)
云南省中青年学术与技术带头人培养费(2004PY01-5)
昆明理工大学人才培养费
云南昆明味知农产品开发有限公司资助
关键词
洋桔梗
种子
叶片
离体培养
Eustoma grandiflorum
Seed
Leaf
In vitro culture
