摘要
抗生素 刺槐 组织培养[目的]为宽叶刺槐进一步的基因转化提供依据。[方法]研究3种抗生素对宽叶刺槐愈伤组织诱导与分化的影响。[结果]在3种抑菌抗生素中，链霉素和噻孢霉素对刺槐外植体离体培养的毒性比羧苄青霉素大，羧苄青霉素只在高浓度才对不定芽的分化有抑制作用。[结论]农杆菌介导刺槐遗传转化时，选用浓度300-400 mg/L的羧苄青霉素抑菌较适合。
[ Objective ] The research aimed to provide references for further study of gene transformation of blacust. [ Method ] Effects of three kinds antibiotics on induction and differentiation of blacust callus in vitro were studied. [ Result ] Among three kinds antibiotics, higher toxicity of cefotaxime and streptomycin were observed having stronger toxicity than that of carbentcillin to the explants,which only showed inhibition to plantlet regeneration under higher concentration. [ Conclusion3 Concentration of 300 -400 mg/L carbentcillJn was optional for the remove of bacterial cells in blacust transformation mediated by Agrobacterium.
出处
《安徽农业科学》
CAS
北大核心
2008年第29期12593-12594,共2页
Journal of Anhui Agricultural Sciences
关键词
抗生素
刺槐
组织培养
Antibiotics
Blacust
Tissue culture
