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纳米羟基磷灰石/聚氨酯支架材料体外的生物活性和降解性 被引量:4

BIOACTIVITY AND DEGRADATION OF HYDROXYAPATITE/POLYURETHANE SCAFFOLD MATERIAL IN VITRO
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摘要 采用纳米羟基磷灰石(hydroxyapatite,HA)粉体与聚氨酯(polyurethane,PU)复合,制备了多孔HA/PU支架材料,通过模拟体液(simulated body fluid,SBF)浸泡试验评估支架材料的降解性和生物活性。用等离子体原子发射光谱仪测定了浸提液中钙离子浓度变化,评估钙离子在支架材料表面的沉积;用电子天平测定了支架材料浸泡前后的质量损失,分析材料的降解性。用X射线衍射、红外光谱分析支架材料浸泡前后结构组成变化,并用扫描电镜观察其表面形貌特征。结果表明:HA/PU支架材料在SBF中,随浸泡时间的延长,材料均有不同程度的降解,PU的降解主要来自于水解。随着PU的降解,表面HA含量增加、溶液中的Ca,P离子过饱和,并与水解的基团成核,在支架材料表面形成一层结晶性较差的钙磷层,使得复合支架随着浸泡时间的延长而表现出最佳的体外生物活性。 Hydroxyapatite/polyurethane (HA/PU) scaffold material was prepared for cartilage repair by the compositing of nano HA powder and PU, and the bioactivity and degradation in vitro of the material were evaluated by immersion testing in a simulated body fluid (SBF) solution for different lengths of time. The Ca ion concentration change on the surface of the HA/PU material soaked in SBF for different time was measured by an inductively coupled plasma-atomic emission spectrometer. The mass loss of the material before and after immersion in SBF solution was determined by electronic balance and the degradation of the material was determined. The constitution and surface appearance of the material were analyzed by X-ray diffraction, infrared and scanning electron microscopy. The results indicate that the HA/PU scaffold material generates a certain of degradation in the SBF solution for different time. This degradation is caused by the hydrolization of PU. With the increase of the hydrolization degree of PU, the HA content on the surface of the material increases, and the concentrations of Ca and P ions in the SBF solution are saturated. Nucleation first occurs in the hydrolyzed group and a calcium phosphorus layer with weak crystallite is formed on the surface of the material. Because of this, the bioactivity of the HA/PU scaffold material in vitro improves with the increase of soaking time.
出处 《硅酸盐学报》 EI CAS CSCD 北大核心 2008年第11期1649-1654,共6页 Journal of The Chinese Ceramic Society
基金 国家“973”计划(2007CB936102)资助项目
关键词 羟基磷灰石/聚氨酯支架材料 模拟体液 降解 生物活性 表征 hydroxyapatite/polyurethane scaffold material simulated body fluid degradation bioactivity characterization
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参考文献18

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