摘要
在实验车间内利用120L的塑料箱作为实验容器,采用自溶面包酵母(Saccharomyces cerevisiae)作为单细胞藻的辅助饵料,研究其在马氏珠母贝(Pinctada martensii Dunker)育苗中的饵料效果,以期解决该贝育苗过程饵料供应不稳定、易培饵料品种小球藻(Chlorella sp.)利用率低的问题。研究结果表明:(1)在温度(30.0±0.5)℃、NaCl质量分数3%、pH6.5～7.0的自溶条件下,用经过18h自溶的酵母投喂D形幼虫,其平均日壳长增长率显著高于其他自溶时间酵母投喂组效果,单独投喂非自溶酵母时D形幼虫不能存活;(2)D形幼虫期单投自溶酵母,幼虫成活率与金藻组没有显著差异(P>0.05),生长速度比金藻组提高8%(P<0.05),说明自溶酵母可以完全替代湛江等鞭金藻(Isochrysis zhanjiangensis Hu﹠Liu),但在壳顶幼虫期和稚贝期单投自溶酵母不能使幼虫和稚贝正常发育和存活;(3)壳顶幼虫期及眼点幼虫至稚贝期投喂"自溶酵母+小球藻(Chlorella sp.)"或"自溶酵母+扁藻[Platymonas subcordiformis(Wille)]",其生长速度及存活率或稚贝育成率均显著高于单投扁藻或小球藻(P<0.05),单投小球藻的饵料效果最差;(4)自溶酵母与小球藻混投对眼点幼虫至稚贝培育效果较单独投喂影响显著,可显著(P<0.05)提高稚贝的育成率、生长速度和耐干露能力。研究表明,使用自溶酵母可以优化马氏珠母贝健康苗种规模繁育中的饵料供应工艺及提高易培饵料品种小球藻的利用价值,从而提高该贝育苗效果。
By using 120 L plastic vessel as experimental container in the nursery workshop, the effects of autolyzed baker yeast as supplemental feed to unicellular algae in the diet of larvae of pearl ouster Pinctada martensii （Dunker）were studied. The aim of this study was to resolve such problems like unstable food supply and low utilization rate of easily cultivated Chlorella sp. in the course of larval cultivation. The results show as follows： （1）The optimum autolysis conditions of baker yeast were 3% salt, pH 6.5-7.0, temperature （30.0- 0.5） ℃ and the autolysis time of 18 h, under which the best feed efficiency could be achieved（P〈0.05） ; as the control, death of larvae appeared using separate feeding of nonautolyzed baker yeast. （2） D-shaped larvae fed on autolyzed baker yeast did not display higher survival rate than those fed on Isochrysis zhanjiangensis （P〉0.05）, but the growth rate of the larvae fed on autolyzed baker yeast was 8% higher than those fed on （P〉0.05） Isochrysis zhanjiangensis. However, umbonate larvae and juveniles couldn＇ t survive when autolyzed baker yeast was provided alone. （3） Umbonate larva and juveniles fed on mixed feed of autolyzed baker yeast and Chlorella sp.or autolyzed baker yeast and Platymonas subcordiformis （Wille） had significantly higher growth and survival rates than those fed on Chlorella sp.or P. subcordiformis alone.The use of mixed autolyzed baker yeast and Chlorella sp. could greatly increase feeding efficiency of Chlorella sp. （4） Fed on mixed feed of autolyzed baker yeast and Chlorella sp. at the stages ranging from eyed-larvae to juvenile, juveniles had higher breeding rate, higher grouth rate and ability to endure drying （P 〈 0.05）. The present optimized food supply protocols for healthy seed production ofP. martensii and made good use of feed such as Chlorella sp., and provided some useful information for commercial production for feed of other shellfish species. [Journal of Fishery Sciences of China, 2008, 15 （6） ： 1 034-1 041]
出处
《中国水产科学》
CAS
CSCD
北大核心
2008年第6期1034-1041,共8页
Journal of Fishery Sciences of China
基金
农业部公益性行业(农业)科研专项(nyhyzx07-047)
关键词
马氏珠母贝
D型幼虫
稚贝
自溶面包酵母
小球藻
饵料效果
Pinctada martensii （Dunker）
D-larvae
juvenile
autolyzed braker yeast
Chlorella sp.
feed efficiency
