摘要
蓝藻水华污染所带来的主要危害是由毒蓝藻向水体中释放多种不同类型的微囊藻毒素（Microcystis，MCs），其中微囊藻毒素MC-LR和MC-RR是我国富营养化水体中最主要的2种藻毒素。为解决对MCs进行深入研究中的MCs纯品缺少问题，迫切需要研究1种有效提取、纯化、制备MCs的方法。以天然水华蓝藻为原料，建立了以甲醇溶液提取、固相萃取和半制备色谱分离为主要步骤的微囊藻毒素提取、纯化和制备的方法。通过优化提取、分离和制备条件，制备了一定量的微囊藻毒素MC-LR和MC-RR高纯度样品。样品经HPLC鉴定分析，纯度可达98％以上，干燥后可得2种微囊藻毒素纯品分别为MC-RR：121．1μg。MC—LR：62．8μg。
A method was established to extract, purify and prepare microcystins （MCs） from natural cyanobacterial bloom by methanol solution extraction, solid phase extraction and separation with semi-prepara- tive chromatography as the main steps. Through optimising the conditions of extraction, separation and purification, certain amount of highly purified MC-LR and MC-RR samples was prepared. The samples were analysed by HPLC, and found to be over 98% in purity. After being dried, 121.1μg of MC-RR and 62.8μg of MC-LR were obtained.
出处
《上海环境科学》
CAS
CSCD
2008年第1期17-20,32,共5页
Shanghai Environmental Sciences
基金
国家高技术研究发展计划项目,编号:2002AA304302
