摘要
目的从银杏叶中分离纯化白果内酯和银杏内酯A、B、C。方法银杏叶提取液经醋酸乙酯萃取、D-101大孔吸附树脂柱和Al2O3柱纯化后得到总内酯提取物,提取物再经两次高速逆流色谱分离制备4种内酯单体。结果25%乙醇热提取、醋酸乙酯萃取、D-101柱、pH4.0的Al2O3柱对总内酯的提纯最终使总内酯质量分数达到44.98%。经高速逆流色谱制备得到的白果内酯和银杏内酯A、B、C最高质量分数分别为98.3%、98.9%、98.8%、98.4%。结论本法简便快速、回收率高。
Objective To separate and purify bilobalide and ginkgolide A, B, and C from Ginkgo biloba leaves. Methods Firstly the extract of G. biloba leaves was purified by extraction of acetic ether, D-101 macroporous resin, and A1203（pH 4） to obtain the crude extract of lactones. Then the crude extract was further purified by high-speed counter-current chromatography （HSCCC） to prepare the monomer of bilobalide and ginkgolide A, B, and C. Results The crude extract of laetones with the purity of 44.98% was obtained by extraction with 25% alcohol, acetic ether, D-101 macroporous resin, and Al2O3（pH 4）. When the crude extract was further purified by HSCCC, the different purity of bilobalide and ginkgolide A, B, and C was got. The maximal purity of bilobalide and ginkgolide A, B, and C was 98.3%, 98. 9%, 98.8%, and 98.4%. Conclusion This method, which is simple and rapid, provides a new way to separate and purify the lactones from G. biloba leaves.
出处
《中草药》
CAS
CSCD
北大核心
2008年第11期1644-1648,共5页
Chinese Traditional and Herbal Drugs
基金
三峡库区生态环境教育部重点实验室科学研究基金项目资助
关键词
银杏叶
白果内酯
银杏内酯A
银杏内酯B
银杏内酯C
高速逆流色谱
Ginkgo biloba L. leaves
hilobalide
ginkgolide A
ginkgolide B
ginkgolide C
high speed counter current chromatography （HSCCC）
