摘要
目的观察缺氧诱导因子-1（HIF-1α）、基质金属蛋白酶-9（MMP-9）和转化生长因子（TGF）-β1在大鼠肝纤维化组织中的表达以及TGF-β1疫苗对这些因子表达的影响。方法二甲基亚硝胺建立大鼠肝纤维化模型，并予以TGF—β1疫苗干预，在实验6周末用免疫组织化学方法检测HIF-1α、MMP-9和TGF-β1在大鼠肝纤维化组织中的表达情况。组间比较采用单因素方差分析。方差齐性者两两比较采用LSD法，方差不齐者进行Dunnet’s T3检验。结果与对照组比较，肝纤维化模型组及TGF-β1疫苗干预组HIF-1α、MMP-9、TGF-β1表达显著增加（P〈0．01），且与肝纤维化程度呈正相关关系（r值分别为0．911、0．814、0．836，P〈0．01）；与肝纤维化模型组比较。TGF—β1疫苗干预组HIF-1α、MMP-9和TGF-β1表达显著降低（t值分别为2．62、4．03、3．43，P〈0．01）。结论肝纤维化发生发展过程中，HIF-1α促进了TGF-β1、MMP-9等基因的转录表达。
Objective To observe the expression and significance of hypoxia inducible factor （HIF）-1α, matrix metalloproteinase （MMP）-9 and transforming growth factor （TGF）-β1 in liver tissues of rats with hepatic fibrosis and the effects of TGF-β1 vaccine on the expressions of HIF-1α, MMP-9 and TGF -β1. Methods The hepatic fibrosis rat model was set up by dimethylnitrosamine （DMN）. The rat model was injected with TGF -β1 vaccine. The expressions of HIF-1α, MMP 9 and TGF-β1 were detected by immunohistochemistry in rat fibrosis tissues after 6 weeks. Results The expressions of HIF 1 α, MMP-9 and TGF-β1 in fibrosis model group and TGF-β2 vaccine group were all significantly higher than control group （P〈0.01）, which was positively correlated with the fibrosis degree （r=0. 911,0. 814 and 0.836, respectively, P〈0.01）. However, the expressions of HIF-1α, MMP 9 and TGF-β1 in TGF -β1 vaccine group were all significantly decreased than fibrosis model group （t= 2.62,4.03,3.43; P〈0.01）. Conclusion During the development of liver fibrosis, HIF-1α promotes the transcriptions and expressions of MMP-9 and TGF-β1.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2008年第11期651-655,共5页
Chinese Journal of Infectious Diseases
