摘要
目的建立用高效液相色谱法测定甜菊糖甙,为生产提供可靠的分析方法,确保产品的质量.方法采用Zorbax—CN（250 mm×4.6 m,5μm）色谱柱;流动相：乙醇-水（35：65）;检测器波长：209 nm;流速：0.8 mL.min^-1;柱温：30℃;以外标法定量,甜菊糖甙标样：自制.结果甜菊糖甙在2～10μg范围内呈良好的线性关系,回归方程为A=9.08×10^3X＋5.30×10^2,r=0.999 9,回收率为100.4%（RSD为0.81%）.结论本方法快速,易操作,准确,可靠,可用于甜菊糖甙的分析.
Objective： To use high -performance liquid chromatography to determine stevia glucosides, to provide reliable analysis method for production and to guarantee the quality of products. Methods： Chromatographic column： Zorbax-CN （250 mm×4.6 m,5μm） ; mobile phase ： Ethyl alcohol - water （ 35：65 ） ; detection wavelength： 209nm; flow rate：0.8mL · min^-1 ;temperature of column is 30℃ ;external standard method is quantitative analysis method, standard sample of stevioside： self - made. Results： The stevia glucosides show a good linear relationship at range of 2 Ixg - 101xg, and the regression equation is A =9.08 × 103X ＋5. 30 × 102,r =0.999 9. The recovery is 100.4% （ RSD was 0.81% ）. Conclusion： This method is fast, accurate, reliable and easy to operate. It can be used in analysis of stevioside.
出处
《辽宁大学学报（自然科学版）》
CAS
2008年第4期355-357,共3页
Journal of Liaoning University：Natural Sciences Edition
关键词
高效液相色谱
甜菊糖甙
色谱样品处理
high-performance liquid chromatography
stevia glncosides
sample treatment.
