摘要
根据热凝胶发酵过程中不同时间和空间对氧传质的不同需求,实施两阶段发酵工艺:第1阶段在种子罐中实施高密度培养,第2阶段在2级发酵罐中稀释细胞密度,使微生物直接进入产胶阶段,同时解除氧传质对产热凝胶的限制作用,达到高强度发酵生产热凝胶的目的。通过对两阶段发酵工艺的构建和优化,结果表明该工艺较好地满足菌体生长和热凝胶合成对营养和氧传质的不同要求,热凝胶产量达到66 g/L,相对于分批发酵工艺产胶量提高了106%,比产胶速率提高了20%,并且在菌体生长阶段发酵规模比分批发酵缩小了75%,因此节约了能量,降低了功率消耗。
A two- stage fermentation process was established based on the different oxygen transfer demand during the curdlan fermentation. Firstly, high cell density culture was performed in the inoculant fermenter. Secondly, inoculant cultures were diluted into the next larger fermenter for curdlan production, in which glucose was fed as sole carbon source. The results showed that the fermentation strategy could meet the oxygen and nutrients requirements for cell growth and curdlan production. A yield of 66 g/L curdlan was achieved, higher 106% than that of batch fermentation. The specific production rate was improved by 20% and the fermentation volume was reduced by 75% than those of batch fermentation. Meanwhile, the power consumption was reduced obviously in the cell culture stage.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2008年第5期39-44,共6页
Journal of Food Science and Biotechnology
基金
国家863计划项目(2006AA02Z207)
关键词
粪产碱杆菌
热凝胶
两阶段发酵
高密度培养
Alcaligenes faecalis
curdlan
two-stage fermentation
high cell density culture
