摘要
对实验室筛选出产β-葡萄糖苷酶的黑曲霉进行辐射诱变处理得到高产酶突变菌株,同时采用单因素和正交实验对黑曲霉产酶培养基进行优化研究。结果表明:经过X射线辐射诱变的黑曲霉产的β-葡萄糖苷酶活力提高了18.86 U/mL。该菌株最适宜产酶的培养基为(质量分数%):麸皮与玉米粉(1∶1)1.5、(NH4)2SO40.15、KH2PO40.2、吐温80 0.1,所得酶活力最大可达到59.86 U/mL。
In this study, Aspergillus niger mutant with high β-glucosidase was obtained by radiation. Single factor and orthogonal test were developed to optimize the culture medium for β- glucosidase production. Only 18. 86 U/mL β-glucosidase was achieved after X-ray radiation mutation. The optimum composition of medium were listed, bran and maize power （1 ： 1） 1.5%, （NH4）2SO4 0.2%, KH2PO% 0.2%, Tween 80 0. 1%. the highest β-glucosidase activity were achieved at 59.86 U/mL with the optimum medium.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2008年第5期124-127,共4页
Journal of Food Science and Biotechnology
关键词
Β-葡萄糖苷酶
黑曲霉
辐射诱变
培养基
优化
β-glucosidase
Aspergillus niger
mutation breeding by radiation
culture medium
optimization
