摘要
目的探讨TNF-α mRNA和TGF-β1 mRNA在慢性阻塞性肺疾病(COPD)模型大鼠气道组织的表达及前列腺素E1(PGE1)对COPD大鼠模型气道炎症的干预作用。方法30只Wistar大鼠随机分为3组:对照组、模型组和治疗组(n=10)。以气道内滴注脂多糖(LPS)和熏烟法建立COPD模型,治疗组在第2-28天(第15天除外)在熏烟前将前列腺素E1脂微球载体制剂2ml溶于8ml生理盐水,按2.5ml/kg从尾静脉注入大鼠体内,第29天对大鼠行肺功能测定,然后处死大鼠,收集支气管肺泡灌洗液(BALF),对炎症细胞行总数和分类计数,对大鼠肺组织行病理学检查,逆转录聚合酶链反应(RT-PCR)测定肺组织中TGF-β1 mRNA和TNF-α mRNA的表达水平。结果所建COPD模型的病理学改变符合人类COPD的特点。治疗组肺组织的病理改变较COPD模型组明显减轻但未恢复正常。治疗组BALF中白细胞总数及中性粒细胞数、肺组织TNF-α mRNA、TGF-β1 mRNA的表达下降,差异有统计学意义(P<0.01)。结论TNF-α mRNA和TGF-β1 mRNA在COPD大鼠气道组织的表达明显增多,前列腺素E1能抑制COPD大鼠气道TNF-α mRNA和TGF-β1 mRNA的表达,从而对COPD的气道炎症起到一定的防控效应。
Objective To evaluate the expression of TGF-β1 mRNA and TNF-α mRNA in airway tissues of rats with chronic obstructive pulmonary disease（COPD ）, and to evaluate the therapeutic effects of prostaglandin E1 on airway inflammation in rats with COPD. Methods Thirty Wistar rats were randomly divided into three groups, control group, model group and treatment group. The models of COPD were established by intratrachel instillation of lipopolysaccharide（LPS）and daily exposured to cigarette smog. From day 2 to day 28（except daylS） ,2 ml Lipo-Prostaglandin E1 （PGE1）was dissolved into 8 ml normal saline,and then were intravenously injected into the rats in treatment group at a dose of 2.5 ml/kg from vena candalis before exposure to smog. At day 29,lung function was detected, and then the rats were killed. Total and differential cell counts in bronchoalveolar lavage fluid（BALF）were determined, and the pathomorphological changes in the lung were observed. The expression of TGF-β1 mRNA and TNF-α mRNA of lung tissues was detected by RT-PCR. Results The pathological changes in COPD rat models were coincident with that in humans. Pathological changes of lung tissues in treatment group were better than that in model group, but worse than in control group. The total cell counts and neutrophil counts in BALF in treatment group were less than those in model group（P〈0.01）. The expression of TNF-α mRNA and TGF-β1 mRNA in lung tissues were lower than in model group（ P 〈0.01）. Conclusion. The results indicate that PGE1 may have preventive and therapeutic effects on airway inflammation of COPD by decreasing the expression of TGF- β1 mRNA and TNF-α mRNA.
出处
《山西医科大学学报》
CAS
2008年第11期968-971,共4页
Journal of Shanxi Medical University
基金
2008年山西省研究生优秀创新项目(20081055)
