摘要
目的探讨牡蛎酶解液的抗氧化活性。方法用普鲁士蓝反应法测定牡蛎酶解液还原能力,在DPPH体系、Fenton体系和邻苯三酚自氧化体系中分别检测牡蛎酶解液清除二苯代苦味酰自由基(DPPH.)、羟基自由基(.OH)和超氧阴离子(O2-.)的能力,并考察牡蛎酶解液在Fe2+诱发卵黄脂蛋白过不饱和脂肪酸(PUFA)过氧化体系中的抗氧化作用。结果牡蛎酶解液清除DPPH.和.OH的EC50分别为7.313和1.330 mg/ml;牡蛎酶解液(17.800 mg/ml)对超氧自由基和卵黄脂蛋白脂质过氧化的抑制率分别为19.86%和82.97%。结论牡蛎酶解液具有显著的清除DPPH.和.OH作用,且活性与剂量呈正相关,并对卵黄脂蛋白脂质过氧化也显示出了较强的抑制作用。
Objective To study the antioxidant activities of enzymatic hydrolysate from oyster. Methods Its reducing power was detemined by the Pursian blue reaction. Scavenging effects on DPPH radical, hydroxyl free radical（ · OH） produced from Fenton system and superoxide anion free radical （O2 ^-· ） produced from the self oxidation of pyrogallol were measured. In addition, the antioxidant effect on peroxidation of polyunsaturated fatty acid from lipoprotein induced by iron was studied. Results The concen- tration of scavenging 50% （EC50） DPPH · and · OH of enzymatic hydrolysate from oyster were 7. 313 mg/ml and 1. 330 mg/ml respectively. The inhibition rate of enzymatic hydrolysate （17. 800 mg/ml） on the pyrogallol antioxidation and lipid peroxidation reached 19.86% and 82.97% respectively. Conclusion Enzymatic hydrolysate from oyster has evident effects of scavenging DPPH · and · OH in a concentration - dependent manner. In addition, enzymatic hydrolysate also has inhibitory effect on the peroxidation of PUFA.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第11期2674-2676,共3页
Lishizhen Medicine and Materia Medica Research
基金
广西壮族自治区科学研究与技术开发计划项目(桂科攻0718003-2-7)
关键词
牡蛎
酶解
自由基
抗氧化
Oyster
Enzymatic hydrolysis
Free radicals
Antioxidant activity
