摘要
目的了解江浙地区两所医院铜绿假单胞菌(PAE)连续分离株的耐药性和16S rRNA甲基化酶、氨基糖苷类修饰酶基因存在情况。方法K-B法检测PAE连续分离株的耐药性,PCR方法检测5种16S rRNA甲基化酶(rmtAr、mtB、rmtC、rmtD、armA)和6种氨基糖苷类修饰酶基因(aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Ⅰb、aac(6′)-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ)。结果两所医院PAE对头孢哌酮/舒巴坦、亚胺培南、美罗培南与阿米卡星药物敏感性A院为74.2%、80.0%、82.9%、68.5%,B院为90.0%、50.0%、50.0%、95.0%;β-内酰胺类药物、环丙沙星、复方新诺明耐药率极高,两所医院PAE均未检出16S rRNA甲基化酶。结论不同医院PAE氨基糖苷类修饰酶基因检出率可不同。
OBJECTIVE To investigate the drug-resistance and the existence of genes in 16S rRNA methylases and aminoglycoside modifying enzymes in strains continuously isolated from Pseudornonas aeruginosa （PAE） in two hospitals of Jiangsu and Zhejiang Provinces. METHODS The drug-resistance of the strains continuously isolated from PAE was detected with K-B test, five kinds of genes in 16S rRNA methylases （rmtA, rmtB, rmtC, rmtD and armA） and six kinds of aminoglycoside modifying enzymes [aac（3）- Ⅰ , aac（3）- Ⅱ , aac（6＇）- Ⅰ b, aac（6＇）- Ⅱ , ant（3＂）-Ⅰ and ant（2＂）-Ⅰ ] were detected by PCR. RESULTS The strains were just sensitive to cefoperazone/ sulbactam, imipenem, meropenem and amikacin （hospital A.. 74.2%, 80.0%, 82. 9% and 68. 5% ;hospital B.. 90.0%, 50.0%, 50.0% and 95.0%, respectively）. There was a high rate in the drug-resistance to β-laetamase medicines, ciprofloxacin and sulfamethoxazole co. Genes in 16S rRNA methylases were not detected from PAE strains in the two hospitals. CONCLUSIONS The rates of genes in aminoglycoside modifying enzymes detected from strains in continuously isolated from PAE are different in different hospitals.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第11期1501-1504,共4页
Chinese Journal of Nosocomiology
关键词
铜绿假单胞菌
甲基化酶
氨基糖苷类修饰酶
基因
Pseudornonas aeruginosa
Methylases
Aminoglycoside modifying enzymes
Genes
