结缔组织生长因子在兔眼结膜下组织创伤愈合过程中的表达被引量：6

The expression of connective tissue growth factor in rabbit subconjunctival tissue wound healing

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摘要目的观察结缔组织生长因子(CTGF)在兔眼结膜下组织创伤愈合过程中的表达,探讨其在结膜下组织瘢痕形成过程中的作用。方法新西兰白兔15只,随机选取一眼建立结膜瘢痕化模型,另一眼作为对照,分别于术后1、5、9d摘除眼球,剪取手术部位结膜及结膜下组织。将所取标本分为两部分,一部分行免疫组织化学检测;另一部分用于半定量逆转录聚合酶链反应(RT-PCR)。结果免疫组织化学染色显示,术后第1d,CTGF染色增强;术后5d时,染色进一步增强;术后9d阳性染色开始减弱,但仍较正常组高。半定量RT-PCR结果显示,术后第1d CTGF mRNA的表达升高,在术后5d时表达最强,至9d时表达水平下降,但仍较对照组高,差异具有统计学意义(P<0.01)。结论在创伤愈合过程中兔眼结膜下组织中CTGF蛋白及其mRNA表达同期增高,提示阻断或抑制CTGF的基因表达或蛋白合成,可能为防止青光眼滤过手术的失败提供一个新的治疗途径。 Objective Connective tissue growth factor （CTGF） is one of the most important factors in tissue wound healing.This study was designed to evaluate the role of CTGF in subconjunctival tissue wound healing. Methods Operation was performed on laterial eyes of 15 New Zealand rabbits,and the fellow eyes without operation were as controls.Rabbits were killed on day 1,5 and 9 after operation.Conjunctiva and subconjunctiva tissue was harvested for histopathological and immunochemical examination under the light microscope.The expressions of CTGF and GAPDH were detected by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results Immunohistochemistry positive stain showed the brown-yellow particles in the cytoplasm.Positive expression of CTGF in fibroblasts was enhanced 1 day after surgery in comparison with control eyes.In 5 days after surgery,the stain of CTGF was stronger obviously than 1 day group.Nine days later,the stain intensity of CTGF was weakened but was still stronger than normal eye.RT-PCR results indicated that CTGF mRNA was up-regulated 1 day and peaked in 5 days and began to descend in 9 days after surgery but was still higher than the control eyes,showing a statistically significant difference （P〈0.01）. Conclusion During the progress of rabbit subconjunctival tissue wound healing,the expression of CTGF increases in both protein and mRNA levels.It suggests that inhibition of CTGF synthesis may have a preventing effect on bleb failure and improving effect on glaucoma filtration surgery.

作者姚琳王大博白海青靳睿

机构地区潍坊市益都中心医院眼科青岛大学医学院附属医院眼科邯郸中心医院眼科

出处《眼科研究》 CSCD 北大核心 2008年第11期837-840,共4页 Chinese Ophthalmic Research

关键词结缔组织生长因子青光眼瘢痕化 connective tissue growth factor glaucoma scarring

分类号 R779.1 [医药卫生—眼科]

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1Fourmans S. Effects of aminopropionitrile on glaucoma filter blebs in rabbits [ J ]. Ophthahnic Surg, 1998,19 (9) : 649 - 652.
2陈松.青光眼滤过性手术中抗瘢痕形成药物的研究近况[J].国外医学（眼科学分册）,1994,18(4):193-201. 被引量：55
3Nakanishi T, Kimura Y, Tamura T, et al. Cloning of a mRNA preferentially expressed in chondroeytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA [ J ]. Biochem Biophys Res Commun, 1997,234( 1 ) : 206 -210.
4Bradham DM, Igarashi A, Potter RL, et al. Connective tissue factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10 [J]. Cell Biol,1991,114: 1285 - 1294.
5Grotendorst GR. Connective tissue growth factor: a mediator of TGF-beta action on fibroblasts [ J ]. Cytokine Growth Factor Rev, 1997,8 (3) : 171 - 179.
6Blom IE,van Dijk AJ, Wieten L, et al. In vitro evidence for differential involvement of CTGF and PDGF-BB in mesangial response to injury[ J ]. Nephrol Dial Transplant ,2001,16:1139 - 1148.
7Gore-Hyer E, Shegogue D, Maekiewicz M, et al. TGF-beta and CTGF have overlapping and distinct fibrogenic effects on human renal cells[ J]. Am J Physiol Renal Physiol,2002,283: F707 -716.
8Weston BS, Wahab NA, Mason RM. CTGF mediates TGF-beta-induced fibronectin matrix deposition by upregulating active alpha5 betal integrin in human mesangial cells[ J]. J Am Soc Nephrol,2003 .14: 601 - 610.
9Julie T, Gregory S, Timothy D, et al. Mediation of transforming growth factor-β1 -stimulated matrix contraction by fibroblasts [ J ]. Am J Pathol, 2003,163 : 2043 - 2052.
10Chudgar SM, Deng P, Maddala R, et al. Regulation of connective tissue growth factor expression in the aqueous humor outflow pathway [ J ].Mol Vis,2006,12:1117 - 1126.