摘要
目的探讨致孔剂NaCl粒径和比例、变性剂和聚乙烯醇（PVA）等因素对基因重组蛛丝蛋白-PVA复合支架材料形态及性能的影响。方法基因重组蛛丝蛋白溶解于98％甲酸，采用冷冻干燥粒子滤沥法制备重组蛛丝蛋白-PVA复合多孔支架；采用扫描电子显微镜观察支架的形态；采用单纤维强力试验机测试支架机械性能。结果乙醇作变性剂制得的多孔支架力学性能较好，支架的断裂应力、断裂比强度均提高5倍以上，断裂伸长率可达12．21％。以粒径〈500tzm的NaCl为致孔剂制得的多孔支架力学性能较好。高分子材料PVA能明显改善重组蛛丝蛋白多孔支架的性能。结论重组蛛丝蛋白-PVA复合支架材料有望在组织工程领域得以应用。
Objective To investigate the effects of many factors such as porogen, denaturant and polyvinyl alcohol （PVA） polymer on the structure and properties of recombinant spider silk protein-PVA composite scaffold material. Methods The silk fibrous protein solution was prepared by dissolving the recombinant spider silk protein in 98% formic acid. Recombinant spider silk protein-PVA composite porous scaffolds were prepared by freeze-drying and particle-leaching method. Scanning electron microscope was used to observe the conformation of the scaffolds. Single strand strength testing machine was used to test the mechanical property of the scaffolds. Results Mechanical property of the scaffolds was better with ethanol as denaturant. Breaking stress and break specific tenacity enhanced by over 5 times. Breaking elongation ratio could reach 12.2l %. Using NaCl （φ〈 500μm） as porogen, or the introduction of PVA could improve the performances of porous scaffold evidently. Conclusion Recombinant spider silk protein-PVA composite porous scaffolds will have a prospective application in tissue engineering.
出处
《中华生物医学工程杂志》
CAS
2008年第3期189-191,共3页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金（30570956）
福建省发改委项目（2006-781） 感谢西安工程科技学院姚穆院士对本实验提供材料测试的支持和帮助
关键词
重组蛛丝蛋白
冷冻干燥
聚乙烯醇
支架
Recombinant spider silk protein
Lyophilized
Polyvinyl alcohol
Scaffolds
