摘要
本文对我国允许使用TBHQ的食品样品的前处理方法进行详细研究,优化了样品处理及分析条件,建立了液相色谱-荧光检测器法(HPLC-FD)对食品中TBHQ进行定量检测的方法:样品经乙腈提取其中的TBHQ,经反相C18色谱柱分离后,用荧光检测器检测,以保留时间定性,面积外标法定量。最低检出限0.05mg/L,线性范围0.1 mg/L～10 mg/L。本方法灵敏度高,能简便、快速、准确地检测食品中抗氧化剂TBHQ。
In this paper, the preprocessing method that China allows the use of TBHQ in food samples was studied in detail. A HPLC - FD analytic method was developed for determination tert - butylhydroquinone （TBHQ） in food. The optimized conditions of determination were studied. The sample was extracted by acetonitrile after crush- ing and picking - up TBHQ. The clear liquid was separated by C18 and determinated by HPLC - FD and quantified by the ratio of sample area between standard sample area. This method can avoid the interference and has better selectivity than the existing methods. The detection limits of this method are 0.05 mg/L and the range of determination was 0.1 mg/L - 10 mg/L. This method is sensitive, and can effectively detect TBHQ accurately in food.
出处
《现代科学仪器》
2008年第5期58-60,72,共4页
Modern Scientific Instruments
