摘要
目的通过过继转移日本血吸虫感染鼠树突状细胞(DC),探讨DC在抑制过敏性哮喘中的作用及机制。方法用CD11c+磁珠分离纯化日本血吸虫感染鼠及正常鼠DC亚群CD11c+细胞。将15只BALB/c小鼠随机分为3组,A组为过继转移日本血吸虫感染鼠DC并诱发过敏性哮喘组,B组为过继转移正常鼠DC并诱发过敏性哮喘组,C组为单纯过敏性哮喘组。A、B两组小鼠经尾静脉过继转移1×106DC,2 h后A、B、C 3组均开始诱发哮喘。4周后处死小鼠,收集肺泡灌洗液,检测细胞因子IL-4I、L-5及IFN-γ水平;取小鼠肺组织作病理切片,观察其炎症变化。结果与B、C组比较,A组肺部炎症明显减轻,按Underwood标准,A、B、C 3组总评分分别为5.00±1.58、11.40±2.07和13.20±2.86,差异有统计学意义(P<0.05)。A、B、C组小鼠肺泡灌洗液IL-4水平分别为(23.25±1.57)pg/ml、(40.70±3.28)pg/ml和(65.23±4.84)pg/ml,差异有统计学意义(P<0.05);IL-5水平分别为(16.20±4.11)pg/ml、(41.41±7.14)pg/ml和(64.75±16.96)pg/ml,IFN-γ水平分别为(6.60±2.34)pg/ml(、6.94±2.19)pg/ml和(5.87±2.44)pg/ml,差异无统计学意义(P>0.05)。结论日本血吸虫感染鼠DC通过影响受体鼠Th2细胞因子的分泌明显抑制过敏性哮喘的发生。
Objective To study the inhibition of allergic asthma by adoptive transfer of dendritic cells （DC） from Schistosoma japonicum-infected mice and its mechanisms. Methods DC from S. japonicum-infected mice and naive were respectively isolated and purified using CD11c^＋ microbeads. Fifteen BALB/c mice were divided into three groups randomly. 106 DC of S. 7aponicum-infected mice （isDC） were injected into the tail vein of each mouse in Group A, while 106DCs of naive （inDC） were injected into the tail vein of each mouse in Group B. Two hours after injection, mice in Group A, B and C were sensitized and challenged with ovalbumin to induce asthma. Four weeks later, all the mice were killed, and bronchoalveolar lavage fluids （BALF） were collected for measurement of various cytokine （IL-4, IL-5 and IFN-7） by ELISA, and the left lungs were cut for histopathological analysis. Results Compared with mice in Group B and C, pulmonary inflammation of mice in Group A diminished markedly. According to Underwood＇s standard, average total scores of Group A, BandCwere5.00±1. 58, 11.40±2.07 and 13. 20±2.86 respectively（P〈0.05）. Moreover, the levels of IL-4 of mice in Group A, B and C were （23. 25±1.57） pg/ml, （40.70±3.28） pg/ml and （65.23±4.84） pg/ml respectively （P〈0.05）. The levels of IL-5 of mice： Group A, B and C were （16.20±4.11） pg/ml, （41. 41±7.14） pg/ ml and （64.75±16.96） pg/ml respectively （P〈0.05）. The levels of IFN-7 of mice in Group A, B and C were （6.60± 2.34） pg/ml, （6.94±2.19） pg/ml and （5.87±2.44） pg/ml respectively （P〉0.05）. Conclusion DC of S. japonicure infected mice can significantly inhibit allergic asthma through influence of Th2 cytokine production by recipient mice.
出处
《中国病原生物学杂志》
CSCD
2008年第11期832-834,F0004,共4页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.30671830)
