摘要
目的:探讨三叶因子3(trefoil factor 3,TFF3)和CD147在不同胃黏膜病变中的表达与间质微血管(microvessel density,MVD)值的关系.方法:利用组织芯片技术制作302例的组织芯片,同时采用S-P免疫组化方法检测TFF3、CD147和CD34表达.结果:萎缩性胃炎、不典型增生和胃癌各组TFF3表达均高于浅表性胃炎和正常组(48.3%,51.9%,41.7%vs13.3%;48.3%,51.9%,41.7%vs3.6%,均P<0.01);胃癌CD147表达和MVD高于正常胃黏膜、浅表性胃炎、萎缩性胃炎和不典型增生(78.9%vs14.3%,43.3%,51.2%,59.3%;31.86±9.92vs26.10±6.82,24.74±5.49,20.77±6.87,14.95±6.28,均P<0.05),浅表性胃炎CD147表达和MVD与正常胃黏膜之间差异显著(43.3%vs14.3%;20.77±6.87vs14.95±6.28,均P<0.05).TFF3、CD147表达和MVD与胃癌淋巴结转移和TNM分期有关(P<0.05),TFF3表达与胃癌组织学类型有关(P<0.05),CD147表达与胃癌分化程度和胃癌浸润深度有关(P<0.01),MVD与胃癌浸润深度有关(P<0.05).TFF3、CD147阳性表达的MVD高于阴性的(35.47±9.41vs29.27±9.50;33.33±9.62vs26.40±9.17,P<0.01).TFF3和CD147表达与MVD呈显著正相关(r=0.323,r=0.279).TFF3(+)/CD147(+)者在深度浸润(T3-4)、临床分期TNMⅢ-Ⅳ、淋巴结转移的比率和MVD最高,且明显高于TFF3(-)/CD147(-)者(P<0.05).结论:TFF3、CD147和CD34在胃黏膜癌变和癌变后的恶性演进过程中起重要作用,可作为胃癌的早期诊断和预测胃癌发生转移的重要指标.
AIM： To investigate expression of trefoil factor 3 （TFF3） and CD147 in gastric mucosa, and their correlation with microvessel density （MVD） in carcinogenesis of gastric mucosa.
METHODS： Three hundred and two sample tissues were prepared using tissue microarray. At the same time, S-P immunohistochemical methods were applied to detect expression of TFF3, CD147 and CD34.
RESULTS： TFF3 expression was significantly higher in atrophic gastritis, atypical hyperplasia and gastric cancinoma than in normal controls or in superficial gastritis （48.3%, 51.9%, 41.7% vs 13.3%; 48.3%, 51.9%, 41.7% vs 3.6%, all P 〈 0.01）. CD147 expression and MVD were higher in gastric cancinoma than in normal controls, superficial gastritis, atrophic gastritis or in atypical hyperplasia （78.9% vs 14.3%, 43.3%, 51.2%, 59.3%; 31.86 ± 9.92 vs 26.10 ± 6.82, 24.74 ± 5.49, 20.77 ± 6.87, 14.95 ± 6.28, all P 〈 0.05）. There was significant difference in CD147 expression and MVD between superficial gastritis and normal controls （43.3% vs 14.3%; 20.77 ± 6.87 vs 14.95 ± 6.28, all P 〈 0.05）. TFF3 and CD147 expression and MVD were correlated with lymph node metastasis and TNM staging （all P 〈 0.05）. TFF3 expression was correlated with histological type （P 〈 0.05）, CD147 expression was correlated with tumor differentiation （P 〈 0.01）. CD147 expression and MVD were correlated to depth of invasion （P 〈 0.05）. MVD with positive expressions of TFF3, survivin and CD147 was higher than that with negative expression （35.47 ± 9.41 vs 29.27 ± 9.50; 33.33 ± 9.62 vs 26.40 ± 9.17, all P 〈 0.01）. The expression of TFF and CD147 and MVD were positively correlated（r = 0.323, r = 0.279）. Rate of the deeper tumor invasion （T3-4）, TNM categories （TNMⅢ-Ⅳ）, lymph node metastasis and MVD reached the highest in TFF3（＋）/CD147（＋） and were markedly higher in TFF3（＋）/CD147（＋） than TFF3（-）/CD147（-） （P 〈 0.05）.
CONCLUSION： TFF3, CD147 and CD34 may play important roles in carcinogenesis and gastric carcinoma development of gastric mucosa. They can be used as important markers for early diagnosis and reflecting metastasis in gastric cancinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第32期3631-3636,共6页
World Chinese Journal of Digestology
基金
福建省漳州市科技局资助项目
No. Z07020~~
关键词
胃肿瘤
组织芯片
免疫组化
三叶因子3
CD147
微血管密度
血管形成
Stomach neoplasms
Tissue microarray
Immunohistochemisty
Trefoil factor 3
CD147
Microvessel density
Angiogenesis
