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禽呼肠孤病毒血清学相关性及S3基因序列同源性分析 被引量:1

Serologic correlation and sequence homology of S3 gene in four avian reovirus isolates
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摘要 采用血清学交叉中和试验方法,分析了4株禽呼肠孤病毒株CL(鸡源禽呼肠孤病毒)、YH和YB(鸭源禽呼肠孤病毒)及S1133(禽呼肠孤病毒标准株)之间抗原的相互关系;并对CL株的S3基因进行克隆测序,用DNAsis和Prosis等分析软件进行序列比较及推导氨基酸序列分析.结果表明:4株禽呼肠孤病毒株之间具有共同的群特异抗原,但不同病毒株间存在中和抗原位点的不对称性;血清学相关性与S3基因序列及其推导的σB蛋白氨基酸序列同源性不一致,表明禽呼肠孤病毒诱导机体产生特异性中和抗体的病毒蛋白不止σB蛋白一个,σB氨基酸序列同源性与血清学相关性没有必然的直接联系. Four strain avian reovirus, CL (isolated from chicken), YH (isolated from muscovy duck), YB (isolated from muscovy duck) and S1133 (standard strains) were examined in cross-neutralization tests using corresponding antiserum, and the antigen relationship of avian reovirus was analyzed. At the same time, the 53 gene of DRV-CL was cloned and sequenced, and the amino acid sequence of polypeptide displayed on phage was deduced. Our results show that the four virus isolates share related antigenic sites. Interestingly the antigenic epitopes among the isolates exhibit unsymmetrical characteristics. There was inconsistence between serologic correlation and the sequence homology of (rB protein genes. This work provides evidence for the existence of induce specific antibodies other than (rB protein in avian, and the sequence homology of (rB protein genes has no direct inevitable relation to serologic correlation.
出处 《福建农林大学学报(自然科学版)》 CSCD 北大核心 2008年第6期625-630,共6页 Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金 福建省自然科学基金资助项目(2002F003 Z0516012)
关键词 禽呼肠孤病毒 交叉中和试验 S3基因 avian reovirus cross-neutralization test 53 gene
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  • 1[2]U.Heffels-Redmann,et al., 1992.Structural and biological characteristics of reoviruses isolated from muscovy ducks (cairina moschata). Avian pathol. 21,481~491.
  • 2[3]Gustavo,B.,et al. , 2001.The avian reovirus genome segment S1 is a functionally tricistronic gene that expresses one structural and two nonstructural proteins in infected cells,Virology 290,181~191.
  • 3[4]Gaelle Kuntz-Simon,et al. ,2002.Muscovy duck reovirus δ C protein is atypically encoded by the smallest genome segment,J.Gen. Virol.83,1189~1200.
  • 4[5]Le Gall-recule,G., et al.,1999.Molecular characterization and expression of the S3 gene of muscovy duck reovirus strain 89026, J.Gen.Virol.80,195~203.
  • 5[10]Hsiao,j.,et al.,2OO2.Cloning,expression,and characterization of avian reovirus guanylyltransferase,Virolgy296(2) :288~299.
  • 6[11]Liu,H. L.,1997. In situ detection of reovirus in formalin-fixed,paraffin-embedded chicken tissues using a digoxgenin-labeled Cdna probe,Avian Disease,41:447~451.
  • 7[12]Hsien,SH.Y.,et al.,1998.Development and characterization of a nucleic acid probe for avian reoviruses,Avian path.27:423~426.
  • 8[14]Matinez-costas,J.,et al.,1997.Protein architecture of avian reovius S1133 and identification of the cell attachment protein,J.Virol.71,59~64.
  • 9[15]Liu,H. L.,1997.Amplification,cloning and sequecing of the sigma C-encoded gene of avian reovius,J.Virol.method, 163:203~208.
  • 10Tang K N,Avian Diseases,1987年,31卷,584页

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