摘要
采用全菌体蛋白凝胶电泳(SDS-PAGE)对从福州永泰温泉中分离到的60株嗜热菌进行了预筛选,再通过16S rDNA序列分析鉴定出8株代表菌.结果表明全菌体蛋白SDS-PAGE分析是一种简便、快速的菌株筛选方法.
Sixty strains of thermophiles from hot springs in Yongtai County of Fuzhou were discriminated by SDS-PAGE of whole-cell protein of these strains ; 8 representative strains of thermophilic bacteria were identified by analyzing 16S rDNA sequences. The result showed that SDS-PAGE of whole-cell protein was a rapid and simple method for the discrimination of thermophilie bacteria.
出处
《福建农林大学学报(自然科学版)》
CSCD
北大核心
2008年第6期657-661,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省自然科学基金资助项目(2007J0004)
关键词
嗜热菌
分离鉴定
全菌体蛋白凝胶电泳
thermophile
isolation and identification
SDS-PAGE of whole-cell protein