摘要
目的建立一种同时分析牙菌斑中甲酸、乙酸、琥珀酸和乳酸的高效液相色谱法。方法选取正常儿童和唇腭裂儿童各20名,取其全口牙菌斑,离心,取2μL上清液溶于一定体积重蒸水中,于Phenomenex C18柱上用磷酸盐缓冲液-甲醇(97∶3)为流动相,在最大吸收波长处测定4种有机酸的质量浓度。结果甲酸、乙酸、乳酸和琥珀酸线性范围分别为0.110～500、0.049～500、0.047～500、0.084～500μg/mL;检出限分别为0.110、0.049、0.047、0.084μg/mL;相对标准偏差分别为9.5%、7.9%、4.3%、4.2%。对正常和唇腭裂儿童口腔中牙菌斑样品进行检测,甲酸、乙酸、乳酸和琥珀酸样品加标回收率分别为82%～112%、82%～102.5%、90%～115%、80%～110%。结论高效液相色谱法灵敏快速,适合人体口腔牙菌斑中有机酸的检测。
Objective To establish determination method of formic acid, lactic acid, acetic acid and succinic acid in dental plaque with high performance liquid chromatography （HPLC）. Methods After the samples were centrifuged, 2 μL supernatant was transferred to a 1 mL centrifuge tube and diluted in water, then was determined with HPLC. The mixture of phosphate buffer and methanol （97：3） as mobile phase throughout the experiment. The determination of organic acid was performed on Phenomenex C18 column and at their maximum absorption wave. Results The linear ranges of formic acid, lactic acid, acetic acid and succinic acid were 0.110-500, 0.049-500, 0.047- 500, 0.084-500 μg/mL. The detection limits were 0.110, 0.049, 0.047, 0.084 μg/mL. The relative standard derivation were 9.5%, 7.9%, 4.3%, 4.2%. The average recoveries of samples were 82%-112%, 82%-102.5%, 90%- 115%, 80%-110%. Conclusion The method was simple, quick and adapt for analysis of organic acid in dental plaque.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2008年第5期470-472,共3页
West China Journal of Stomatology
基金
国家科技支撑计划课题基金资助项目(07KJB-01)
四川大学校青年基金资助项目(0040405505016)
关键词
高效液相色谱法
有机酸
牙菌斑
high performance liquid chromatography： organic acid： dental plaque
