摘要
目的研究p53上调促凋亡因子（p53up—regulated modulator of apoptosis，PUMA）在胃癌组织中的表达及其与临床病理学指标的关系。方法应用组织芯片和免疫组化Envision方法检测84例胃癌及配对的癌旁正常胃黏膜组织中PUMA和p53蛋白表达；分析PUMA表达与临床病理学指标的关系；分析PUMA表达与p53表达的相关性。结果PUMA在胃癌组织中的阳性率（33％）低于癌旁正常肖黏膜组织（62％），两者之间差异有统计学意义，P〈0．01；PUMA的表达与肿瘤浸润深度、pTNM分期及预后有关（P〈0．05）。p53在胃癌组织中的表达（45．2％）显著高于癌旁正常胃黏膜组织（0），两者之间差异有统计学意义，P〈0．01；PUMA与p53表达有显著的相关性（P〈0．05）。结论PUMA低表达与胃癌浸润深度、胃癌中、晚期及预后不良相关，有可能成为胃癌预后的预测指标．
Objective To investigate the expression of p53 up-regulated modulator of apoptosis （PUMA） and p53 protein and relationship with clinicopathological parameters in human gastric cancers. Methods Primary gastric carcinoma and adjacent normal mucosa were obtained from 84 consecutively enrolled patients undergoing gastric cancer resection at Peking University People＇s Hospital during the period from April 2001 to May 2003. Immunohistochemistry staining was used to measure the expression of PUMA and p53 proteins both in normal and malignant tissues. Results PUMA expression is significantly weaker in primary gastric carcinoma compared to adjacent normal mucosa （P 〈 0. 01 ）. Weak PUMA expression in gastric cancer correlates with depth of tumor invasion （ P 〈 0. 01 ） , advanced pTNM stage （P 〈 0. 05 ） and poor overall survival of patients （P 〈 0. 05）. p53 protein expression in gastric carcinoma is significantly higher than adjacent normal mucosa （P 〈 0. 01 ）. There was a significant negative correlation between the expression of PUMA protein and p53 protein in gastric carcinoma （ P 〈 0. 05 ）. Conclusion PUMA expression in gastric carcinoma is significantly lower compared to adjacent normal mucosa. Weak PUMA expression in gastric cancer correlates with depth of tumor invasion, later pTNM stage and poor overall patient survival.
出处
《中华普通外科杂志》
CSCD
北大核心
2008年第11期859-862,共4页
Chinese Journal of General Surgery
关键词
胃肿痛
基因
p53
细胞凋亡
病理学
临床
Stomach neoplasms
Genes, p53
Apoptosis
Pathology, clinical
