摘要
肝脏卵圆细胞是具有多向分化潜能的干细胞,可转化为胰岛β细胞,为糖尿病的治疗带来了新的希望。目前对于肝脏卵圆细胞的分选主要有Percoll密度梯度离心法、流式细胞技术结合免疫荧光标记单克隆技术(FACS)、免疫磁珠分选技术(MACS)等。分选得到的肝脏卵圆细胞通过一系列转录因子的介导作用可转化为胰岛β细胞,主要的转录因子包括胰腺十二指肠同源盒基因-1(Pdx1)、神经元素3(Ngn3)、神经元分化因子(NeuroD)等,本文从肝脏卵圆细胞的分选和分化等方面对肝脏卵圆细胞向胰岛β细胞分化的研究现状作一综述。
Hepatic oval cells with the potential to differentiate into pancreatic islet β-cells have brought new hope for the treatment of diabetes mellitus. There are several methods to separate hepatic oval cells, such as Pereoll density gradient centrifugation, fluorescence-activated cell sorting technology(FACS) and magnetic activated cell sorting technology(MACS). Separated hepatic oval cells can be differentiated into pancreatic islet β ceils through a series of transcription factors, including Pancreatic and duodenal homeobox factor 1 ( Pdxl ), Neurogenin3 ( Ngn3 ) , Neurogenic differentiation factor 1 (NeuroD) and so on.
出处
《国际内科学杂志》
CAS
2008年第10期593-596,共4页
International Journal of Internal Medicine
关键词
肝脏卵圆细胞
胰岛13细胞
糖尿病
分选
分化
Hepatic oval cells
Pancreatic islet β-cells
Diabetes mellitus
Separation
Differentiation