摘要
研究采用酸碱法从梭子蟹壳再利用后的余料中制取甲壳素和壳聚糖的工艺条件。提取甲壳素工艺中,通过单因素试验确定脱钙最优工艺参数为:温度30℃、盐酸浓度6%、酸浸时间4h;脱蛋白最优工艺参数为:温度90℃、NaOH浓度6%、碱煮时间2h。通过正交试验确定制取壳聚糖最优工艺条件为:温度90℃、NaOH浓度50%、时间9h。制取的甲壳素和壳聚糖质量指标均达到食品级标准。
The extraction conditions of chintin from residue ofPortunus trituberculatus shell pretreated with neutral protease were optimized by single test, and the preparation conditions of chitosan from the chintin were optimized by orthogonal test. In extraction process of chintin, the optimal decalcifying conditions are as follows： temperature 30 ℃, HC1 concentration 6% and soaking time with HCl solution 4 h, and the optimal deproteinizing conditions are as follows： temperature 90 ℃, NaOH concentration 6% and cooking time with NaOH solution 2 h. The optimal preparation condition ofchitosan from the chintin are： temperature 90 ℃, NaOH concentration 50% and time 9 h. The extracted chitin and prepared chitosan meet the demands of food grade on quality index.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第11期206-209,共4页
Food Science
基金
广东省教育部产学研结合项目(2007A090302090)
关键词
梭子蟹
梭子蟹壳
甲壳素
壳聚糖
Portunus trituberculatus
shell
chitin
chitosan
