摘要
采用核磁共振、质谱等波谱技术对从海藻真菌B5中分离的化合物P4的结构进行了鉴定,确定该化合物为首次发现的新生物碱类化合物.采用MTT法和流式细胞术就化合物P4体外对肿瘤细胞增殖、凋亡和周期分布的影响进行了初步研究.结果表明,该化合物可显著抑制肿瘤细胞HepG2和Raji的增殖(IC50分别为2.6和10μg/mL),还可明显诱导HepG2细胞凋亡及影响其细胞周期分布.
In this study we reported the isolation of compound P4 from algicolous fungi 135 and the structure identification by NMR and MS spectra. P4 was assign as a new compound. The effects of p4 on the cell proliferation, apoptosis and cell cycle distribution of tumor cell lines in vitro were determined by the methods of MTT and flow cytometric （FCM） DNA analysis. MTT results showed that p4 treatment could significantly inhibit the proliferation of HepG2 and Raji ceils, the IC50 values were 2.6μg/mL and 10μg/mL against the two tumor cell lines, respectively. FCM anatysis revealed that cell apoptosis and G0/G1 arrest could be induced by 20μg/mL p4 treatment for 24 h for HepG2 cell line.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第A02期130-134,共5页
Journal of Xiamen University：Natural Science
基金
国家863计划项目(2006AA09Z410)资助
