摘要
目的研究百仙妇炎清栓中苦参碱的含量测定方法。方法高效液相色谱法,C18色谱柱,流动相：乙腈-0.025 mol.L^-1硫酸铵-十二烷基硫酸钠（30∶70∶0.6）（用硫酸（1→5）溶液调节pH至3.0）;流速：1.0 ml.min^-1,检测波长：210 nm。结果线性范围0.0202-0.2626 g.L^-1,r=0.9999,平均回收率为99.64%,RSD为2.17%（n=6）。结论本方法操作简便,灵敏度高,干扰少,重现性好,可作为该制剂的质量控制方法。
Aim To find a method for determining matrine in baixian fuyanqing shuan by HPLC. Method A C18 column was used. The mobile phase was acetonitrile-0. 025 mol · L^ - 1 ammonium sulfate-sodium laurylsutfate （ 30 ： 70 ： 0. 6 ） （ pH 3.0 by sulfuric acid （ 1 →5 ） ）. The flow rate was 1.0 ml · min^- 1 and UV detection was set at 210 nm. Results The method was linear within the range from 0.0202 to 0.2626 g · L^ -1, r = 0.999 9. The average recovery was 99.64%, and RSD was 2.17% （ n = 6 ）. Conclusions This method is simple, sensitive and reproducible, h can be used for the quality control of the product.
出处
《安徽医药》
CAS
2008年第12期1154-1155,共2页
Anhui Medical and Pharmaceutical Journal
