摘要
目的建立补益舒筋颗粒中黄芪甲苷的含量测定方法。方法利用HPLC-ELSD法,采用Phenomenex-C18色谱柱（4.6 mm×150 mm,5μm）;流动相：乙腈-水（35∶65）;漂移管温度：60℃;氮气气压：150 kPa。结果黄芪甲苷对照品在0.5-10.26μg范围内,进样量与峰面积间的对数值呈良好的线性关系,r=0.999 5,平均回收率98.08%,RSD为1.97%。结论本方法结果准确,重现性好,可作为该制剂的含量测定方法。
Objective To establish the content determination method for astragaloside in Buyishujin Granules by HPLC-ELSD. Methods HPLC with Phenomenex C 18 （150 mm × 4.6 mm,5 μm）column was used. Acetonitrile-water （30： 70）was taken as a mobile phase, flow rate was 1.0mL/min , diift-tube temperature was 60℃, N2 pressure was 150 kPa. Results The linear range of astragaloside was 0.5 - 10.26μg , the correlation coefficient（r） was 0. 999 5, the average recovery rate was 98.08% ,RSD was 1.97%. The method is suitable for the formulate of the quality standard with sufficient accuracy, stability and reappearance. Conclusion The method has characteristics of sufficient accuracy, stability and good reappearance, and can be used for quality control in Buyishujin Granules.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2008年第11期775-776,792,共3页
Journal of Beijing University of Traditional Chinese Medicine
