摘要
目的检测青藏铁路沿线鼠疫菌携带的质粒种类及相对分子质量。方法采用碱裂解、酚一氯仿抽提法提取鼠疫菌质粒，经琼脂糖凝胶电泳进行检测并分析质粒的相对分子质量。结果所检测的18株鼠疫菌具有6×10^6、45×10^6、52×10^6、65×10^6、92×10^6质粒，其中大质粒的变化范围在52×10^6～92×10^6。结论青藏铁路沿线鼠疫菌除具有规范的质粒图谱外，鼠疫菌最大质粒的变化有一定的规律性，具有分类属性，对研究鼠疫自然疫源地空间结构和鼠疫菌的遗传学特性具有重要意义。
Objective To detect the Yersinia pestis plasmid and molecular weight along the Qinghai- Tibet Railway. Methods Yersinia pestis plasmids molecular weight detected and analyzed using alkaline lysis, phenol-chloroform extraction of Yersinia pestis plasmid by agarose gel electrophoresis. Results The 18 Yersinia pestis strains of Qinghai-Tibet Railway contained 6 ×10^6,45 ×10^6,5：2×10^6,65 ×10^6,92×10^6 plasmid, varing in the range of the 52×10^6 - 92 ×10^6. Conclusions The Yersinia pestis of Qinghai-Tibet Railway has a standard plasmid graphics, with the biggest Yersinia pestis plasmid changing in a certain regular degree, which provides significance in the study of plague natural foci of the spatial structure and the genetic characteristics of Yersinia pestis.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2008年第6期595-597,共3页
Chinese Jouranl of Endemiology
基金
“十五”国家科技攻关项目（2004BA718808）
关键词
耶尔森菌
鼠疫
质粒
电泳
琼脂凝胶
Yersinia pestis
Plasmids
Electrophoresis, agar gel
