摘要
目的探讨碘对小鼠产后甲状腺炎（postpartum thyroiditis，PPT）发生、发展的影响。方法44只8周龄雌性C57BL／6J小鼠均饲以低碘饲料（含碘量≤35μg/kg），按体质量随机分成4组：非妊娠实验性自身免疫性甲状腺炎（EAT）组8只，采用猪甲状腺球蛋白加完全弗氏佐剂复制EAT模型，最终存活6只；NI—PPT组（正常碘）、10HI—PPT组（10倍碘）、50HI—PPT组（50倍碘）各12只，复制EAT模型（方法同非妊娠EAT组）后，与性成熟雄鼠交配，分别有7、6、6只小鼠受孕。4组小鼠分别饮用含KI为0．3、0.3、3．0、15．0mg／L的碘水。妊娠母鼠生产4周后，观察4组小鼠甲状腺组织病理改变、血清中甲状腺球蛋白抗体（Tg-Ab）和甲状腺过氧化物酶抗体（TPO-Ab）水平、血清中甲状腺激素（TT3、TT4）水平以及脾脏中IFN-γ和IL-4mRNA的表达水平。结果甲状腺组织病理检查示甲状腺内炎细胞浸润，上皮细胞扁平，滤泡萎缩或破坏。非妊娠EAT组、NI-PPT组、10HI—PPT组小鼠的炎细胞浸润程度均低于50HI—PPT组，组间两两比较，差异有统计学意义（P〈0．05）。非妊娠EAT组、NI-PPT组、10HI-PPT组和50HI—PPT组的血清TPO-Ab水平分别为（14．32±8．85）％、（64．45±10．52）％、（38．46±5．57）％、（90．09±9．98）％，任意两组组间比较，差异均有统计学意义（P〈0．05）；血清Tg-Ab水平分别为（33．74±3．71）％、（29．65±2．06）％、（37．21±3．87）％、（33．87±4．17）％，组间比较差异无统计学意义（F=0．484，P〉0．05）；血清TT3水平分别为（2．47±0．69）％、（1．57±0．25）％、（1．60±0．28）％、（1．82±0．75）％，组间比较差异无统计学意义（F：1．596，P〉0．05）；非妊娠EAT组、NI—PPT组和10HI-PPT组血清中TT4水平[（99．87±5．97）％、（89．13±7．64）％、（91．05±5．82）％]与50HI—PPT组[（66．68±5．47）％]比较，差异均有统计学意义（P〈0．05）。在非妊娠EAT组、NI-PPT组、10HI—PPT组和50HI-PPT组，小鼠脾脏IFN-γ mRNA表达水平分别为1．02±0．10、1．37±0．10、1．39±0．12、1．68±0．06，除NI-PPT组和10HI—PPT组的组间比较之外，其他的任意两组组间比较．差异均有统计学意义（P〈0．05）；IL-4mRNA表达水平分别为0．24±0．05、0．35±0．05、0．49±0．04、0．53±0．06，10HI—PPT组、50HI—PPT组与非妊娠EAT组和NI—PPT组比较，差异均有统计学意义（P〈0．05）。结论妊娠及产后易诱发PPT，增加适量碘的摄人量，可降低PPT的炎症反应，而补碘过量也是诱发或增加产后甲状腺炎发生的重要因素。因此，妊娠及产后补碘应遵循合理和科学的原则。
Objective To explore the effect of iodine on the pathogenesis of postpartum thyroiditis. Methods Forty-four female C57BL/6J mice, 8-week old, fed by low iodine dietary（the concentration of iodine≤35μg/kg）, were randomly divided into 4 groups： non-pregnancy experimental autoimmune thyroiditis （nonpregnancy EAT） group with 8 mice, EAT of mice was induced by immunization with pig＇s thyroglobulin（Tg） in the presence of complete Freund＇s adjuvant. Six mice in non- pregnancy EAT group survived at the end of experiment; normal iodine-PPT（NI-PPT） group, 10-fold high iodine-PPT（10HI-PPT） group and 50-fold high iodine-PPT（50HI- PPT） group with 12 mice in each group. The last 3 groups mice, who received the same immunization schedule as the above, were mated with adult male mice followed by induction of EAT. In the end,7,6 and 6 mice were noticed to be pregnant in each group. All animals were killed 4 weeks after postpartum. Histological severity of thyroid specimens was evaluated. The serum levels of thyroglobulin antibody（Tg-Ab）, thyroid peroxidase antibody （TPO-Ab）, TT3 and TF4 were measured by radioimmunoassay （RIA）. The expression level of IFN-γ/IL-4 mRNA in spleen were assayed by RT-PCR. Results Pathological examination showed the infiltration of inflammatory cells, epithelial cell applanation, follicle atrophy or destruction. The severity of inflammation in non-pregnancy EAT group, NI-PPT group and 10HI-PPT group was less serious than that in the 50HI-PPT group, the difference has statistical significance（P 〈 0.05）. The level of TPO-Ab in non-pregnancy EAT group, NI-PPT group, 10HI-PPT group and 50HI-PPT group was （14.32 ±8.85）%, （64.45 ±10.52）%, （38.46±5.57）% and （90.09± 9.98）%, respectively the difference being statistically significant between any two groups （P 〈 0.05）. There was no statistical difference （F = 0.484,P 〉 0.05） of Tg-Ab among non-pregnancy EAT group [ （33.74 ± 3.71）% ], NI-PPT group [（29.65±2.06）%], 10HI-PPT group [（37.21 ±3.87）%] and 50HI-PPT group [（33.87±4.17）%]. There was no statistical difference（F = 1.596,P 〉 0.05） of TT3 among non-pregnancy EAT group（2.47± 0.69）%, NI-PPT group （1.57± 0.25）%, 10HI-PPT group[ （1.60± 0.28）%] and 50HI-PPT group[ （1.82 ± 0.75）%]. The level of TY4 in 50HI-PPT group [（66.68± 5.47）% ] was lower than that in non-pregnancy group, NI-PPT group and 10HI-PPT group[ （99.87 ± 5.97）%, （89.13 ± 7.64）% and （91.05 ± 5.82）%], the difference being statistically significant（P 〈 0.05）. The expressibn level of IFN-γ mRNA was increasing, being 1.02 ± 0.10,1.37± 0.10,1.39 ± 0.12 and 1.68± 0.06 in non-pregnancy EAT group, NI-PPT group, 10HI-PPT group and 50HI-PPT group. The difference had a statistical significance between any two groups except for NI-PPT group and 10HI-PPT group（P 〈 0.05）. The expression level of IL-4 mRNA in 10HI-PPT group（0.49± 0.04） and 50HI-PPT group（0.53 ±0.06） were all higher than non- pregnancy EAT group（0.24 ± 0.05） and NI-PPT group（0.35 ± 0.05）, the differences being statistically significant （P 〈 0.05）. Conclusions Adequate iodine supplementation during pregnancy and postpartum period is necessary, but iodine excess could induce postpartum thyroiditis. So iodine supplementation during pregnancy and postpartum should be adequate and reasonable.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2008年第6期617-621,共5页
Chinese Jouranl of Endemiology
基金
天津市科技发展计划项目（05YFGDSF02700）
天津市高等学校科技发展基金项目（2004ZD08）
关键词
碘
产后甲状腺炎
自身免疫
Iodine
Postpartum thyroiditis
Autoimmunity
