摘要
背景:传统的组织标本处理程序中包含有多种有毒化学试剂,无论对操作者本人还是对于环境而言都存在着很大的破坏。目的:对传统的组织标本处理程序进行改良,建立组织标本处理的经济环保流程,杜绝相关试剂对操作人员的损害和环境的污染。设计、时间及地点:组织标本流程设计,于1998-02/2008-02在中南大学湘雅三医院病理科完成。材料:标本均系中南大学湘雅三医院常规外检组织标本。冰醋酸、乙醇、松节油、切片石蜡(56～58℃)均为湖南师范大学化工厂产品。自动脱水机ZT-12P购自湖北孝感亚光医用电子技术有限公司。方法:采用无毒的化学试剂替代甲醛和二甲苯,流程为:体积分数为0.7的乙醇冰醋酸液2h、体积分数为0.8的1h、体积分数为0.9的乙醇1h、体积分数为0.95的乙醇Ⅰ2h、体积分数为0.95的乙醇Ⅱ2h、无水乙醇Ⅰ1h、无水乙醇Ⅱ1h、松节油Ⅰ1h、松节油Ⅱ1h、石蜡Ⅰ1h、石蜡Ⅱ1h、石蜡Ⅲ1h;标本取材后放入自动脱水机进行处理。适合于所有人体标本。主要观察指标:经新脱水程序处理的组织标本与传统组织处理结果比较。结果:经改良的环保处理程序处理的标本能顺利切片,且着色均匀,红蓝对比明显。结论:改良的环保处理程序可以避免操作人员接触有毒化学试剂,能完全替代传统程序进行组织标本处理。
BACKGROUND： The traditional tissue processing procedures, which includes a variety of toxic chemical reagents, both for himself and for operating environment there is a lot of damage. OBJECTIVE： To improve the traditional tissue processing procedures, to establish economic processing of tissue sample disposal, and to eliminate damages to the handlers and environment bringing by related reagents. DESIGN, TIME AND SETTING： The tissue sample processing study was performed at the Department of Pathology, Third Xiangya Hospital, Central South University from February 1998 to February 2008. MATERIALS： All specimens were routine surgical specimens of the Third Xiangya Hospital, Central South University. Acetic acid, alcohol, turpentine and paraffin （at 56-58℃） were obtained from Chemical Plant of Hunan Normal University, China. The automatic dehydration machine ZT-12P was purchased from Hubei Xiaogan Yaguang Medical Technology Co., Ltd., China. METHODS： Formaldehyde and xylene were replaced by non-toxic chemical reagents. Processing was as follows, 0.7 volume fraction of ethanol and glacial acetic acid for 2 hours, 0.8 volume fraction of for 1 hour, 0.9 volume fraction of ethanol for 1 hour, 0.95 volume fraction of ethanol 1 for 2 hours, 0.95 volume fraction of ethanol Ⅱ for 2 hours, dehydrated alcohol 1 for 1 hour, dehydrated alcohol Ⅱ for 1 hour, turpentine oil Ⅰ for 1 hour, turpentine oil Ⅱ for 1 hour, paraffin 1 for 1 hour, paraffin Ⅱ for 1 hour, and paraffin Ⅲ for 1 hour. After collecting, samples were put in automatic dehydration machine. Suitable for all kinds of human samples. MAIN OUTCOME MEASURES： To compare the tissue specimens processed by new program with the traditional processing. RESULTS： Specimens treated by improved environmental protection procedures can be sliced up smoothly, and dyed uniformity, which have marked contrast of red and blue. CONCLUSION： Improved environmental protection procedures can avoid handlers exposing to toxic chemical reagents, can entirely replace traditional procedures.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第46期9015-9017,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
