摘要
背景:近期研究提示姜黄素可能是一种抗纤维化制剂。目的:以瘢痕疙瘩成纤维细胞为靶细胞,观察不同浓度姜黄素对瘢痕疙瘩成纤维细胞合成胶原的影响。设计、时间及地点:随机对照实验,于2006-10/2007-11在解放军第四军医大学西京医院烧伤与皮肤外科完成。材料:瘢痕疙瘩患者的手术标本5份,患者均知情同意。治疗方案经医院医学伦理委员会批准。姜黄素为美国Sigma公司产品。方法:瘢痕疙瘩成纤维细胞体外原代培养,待细胞融合后传代,取第3～6代对数生长期的成纤维细胞用于实验。将不同浓度姜黄素(5,10,20μmol/L)分别对瘢痕疙瘩成纤维细胞干预24～48h,以空白组做对照。主要观察指标:蛋白免疫印迹检测姜黄素作用瘢痕疙瘩成纤维细胞后结缔组织生长因子的表达。荧光定量聚合酶链反应检测姜黄素作用瘢痕疙瘩成纤维细胞后Ⅰ、Ⅲ型前胶原及结缔组织生长因子基因的表达。结果:①姜黄素作用瘢痕疙瘩成纤维细胞48h后,结缔组织生长因子蛋白的表达减少,均低于对照组(P<0.01)。②与对照组相比,不同浓度姜黄素干预组Ⅰ、Ⅲ型前胶原及结缔组织生长因子表达均降低(P<0.01),并且Ⅰ型前胶原表达随着药物浓度的增加,有逐渐降低的趋势,成明显的量效关系。姜黄素20μmol/L组Ⅲ型前胶原表达低于姜黄素10μmol/L组,但差异无显著性意义(P>0.05),姜黄素10μmol/L组结缔组织生长因子表达低于姜黄素5μmol/L组,但差异无显著性意义(P>0.05)。结论:姜黄素对体外培养的瘢痕疙瘩成纤维细胞胶原合成有明显抑制作用,其作用机制可能与姜黄素抑制结缔组织生长因子在瘢痕疙瘩成纤维细胞的表达有关。
BACKGROUND： It is reported that curcumin might be a potential antifibrotic drug. OBJECTIVE： To investigate the effects of different concentrations of curcumin on collagen synthesis using keloid fibroblasts as target cells. DESIGN, TIME AND SETTING： Randomized controlled study. The experiment was performed at the Department of Burn and Skin Surgery, Xijing Hospital of Fourth Military Medical University of Chinese PLA from October 2006 to November 2007. MATERIALS： Keloids were derived from 5 cases. The informed consent was obtained from the patients, and the experiment was admitted by the Hospitai Ethics Committee. Curcumin was bought from Sigma, USA. METHODS： Fibroblasts were cultured in vitro, and the Ⅲ-Ⅳ passages of cells at exponential phase of growth were used for experiment. Keloid fibroblasts were stimulated with curcumin （5, 10, 20 μmol/L） for 24-48 hours. Blank group served as controls. MAIN OUTCOME MEASURES： After treatment of curcumin, connective tissue growth factor （CTGF） expressions were detected by Western blot. The expressions of procollagen Ⅰ, procollagen Ⅲ, and CTGF in keloid fibroblasts cultured with curcumin were detected by fluorescent quantitation polymerase chain reaction （PCR）. RESULTS： CTGF expressions in keloid fibroblasts cultured with curcumin for 48 hour were significantly lower than control cells （P 〈 0.01）. The expressions of procollagen Ⅰ, procollagen Ⅲ, and CTGF in keloid fibroblasts cultured with different concentrations of curcumin were significantly lower than control cells （P 〈 0.01）. Moreover, the expressions of procollagen Ⅰ decreased gradually by increased concentration of curcumin in a significant dose-dependent manner. Curcumin 20 μmol/L group showed lower procollagen Ⅲ expression compared with the 10μmol/L group （P 〉 0.05）; Curcumin 10 μ mol/L group showed lower CTGF expression compared with the 5 μmol/L group （P 〉 0.05）; CONCLUSION： Curcumin significantly inhibits collagen synthesis in cultured keloid fibroblasts. The mechanism is probably associated with inhibitory effect of curcumin on CTGF expression in cultured keloid fibroblasts.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第46期9024-9027,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
