摘要
目的:研究O6-甲基鸟嘌呤-DNA甲基转移酶(O6-methylguanine-DNA methyltransferase,MGMT)在肝癌组织中的表达及其与p53的相关性。方法:采用RT-PCR的方法检测40例肝癌组织中MGMT基因的表达水平,并采用免疫组织化学方法检测肝癌组织中p53蛋白的表达。结果:40例肝癌组织中有28例MGMT的表达水平明显低于对照非肝癌组织中,其MGMT mRNA表达的相对量为0.54±0.13,而非肝癌组织组为1.15±0.095。结论:p53蛋白的表达与细胞中MGMT基因的表达有一定的相关性。
Objective ：To explore the expression of MGMT in hepatoeellular carcinoma and the relation between the MGMT and p53. Methods： The exprssion level of MGMT were measured by RT - PCR, and then p53 protein was detected by immunohistoehemistry. Results： Total of 28 cases showed lower levels of MGMT gene in hepatoceUular carcinoma group compared with normal liver. Conclusion： There was correlation between p53 and MGMT gene ex- pression.
出处
《现代肿瘤医学》
CAS
2008年第12期2135-2137,共3页
Journal of Modern Oncology
