摘要
目的比较和探索制备2型糖尿病动脉粥样硬化大鼠模型的不同方法。方法健康雄性Wistar大鼠5只作为对照组,雄性自发性糖尿病模型GK大鼠随机分为高甘油三酯组(12周)、高胆固醇12周组和高胆固醇24周组,每组5只。组织病理学观察各组动脉粥样硬化形成情况。结果四组大鼠血管各层结构基本正常,未见典型动脉粥样硬化病变。免疫组织化学染色显示,四组主动脉壁均无巨噬细胞浸润,中膜及内膜层平滑肌细胞排列规则。结论对于2型糖尿病大鼠动脉粥样硬化模型的制备,这三种方法在Wistar大鼠均不易复制成功。
Aim To compare and investigate the different methods for establishing type 2 diabetic atherosclerosis rat models. Methods 5 Wistar rats were chosen control group and 15 GK rats were randomly divided into three groups： hypertriglyceride diet （12 weeks） group, hypercholesterol diet with vitamin D （12 weeks） group and hyperchlesterol diet with vitamin D （24 weeks） group. Histopathology was used to observe the atherosclerotic lesions. Results There were no obvious morphologic changes in these four groups by light microscope. The macrophage-related antigen expressions in the aorta were not observed in all the four groups. Absence in abnormal expression of SMC-related antigens was shown in all of them. Conclusion All of the three methods were not successful in establishing diabetic atherosclerotic rat models in Wistar rats.
出处
《中国动脉硬化杂志》
CAS
CSCD
2008年第9期707-709,共3页
Chinese Journal of Arteriosclerosis
基金
上海市卫生局科技发展基金(054087)
