摘要
目的探讨苯丙酮尿症患者血清中S-100B蛋白的水平及与苯丙氨酸的关系。方法用ELISA方法测定27例初诊苯丙酮尿症患儿、5例复诊患儿及42例正常儿童血清内的S-100B蛋白水平,用高效液相色谱荧光法测定血清内的苯丙氨酸浓度并对两者进行分析比较。结果苯丙酮尿症初诊患者血清S-100B水平(0.92±0.15μg/L)显著高于复诊者(0.32±0.11μg/L)与健康对照组(0.22±0.09μg/L),差异有统计学显著性意义(P<0.01);复诊者高于健康对照组,差异有统计学意义(P<0.05)。初诊患者血清S-100B的浓度与患者体内苯丙氨酸浓度呈正相关(相关系数0.62,P<0.01)。结论血清S-100B蛋白的水平可作为苯丙酮尿症患者监测脑损伤的指标。
Objective To investigate the change of concentration of serum neural protein S-100B in children with phenylketonuria and the relationship of content between phenylalanine and neural protein S-100B. Methods A total of 27 PKU cases and including 5 return-visit patients were recruited in the study. A group of 42 healthy children were included in the study as a normal control. Their serum samples were analyzed for phenylalanine by HPLC,for neural protein S-100B by enzyme-linked immunosorbent assay（ELISA）. Results The concentration of serum neural protein S-100B in patients with PKU （0.92 ± 0.15μg/L） were significantly high than return - visit patients （0.32 ± 0. 11 μg/L） and normal control （0. 22 ± 0.09 μg/L）. There was also a positive correlation between serum Phe concentrations and serum neural protein S-100B levels in PKU of first visit to a doctor. Conclusion Serum levels of neural protein S-100B could be a useful peripheral marker of brain lesions in patients with PKU.
出处
《现代检验医学杂志》
CAS
2008年第6期110-111,共2页
Journal of Modern Laboratory Medicine
