大鼠海洛因依赖期间睾丸CgA-IR细胞的免疫组织化学研究被引量：1

An Immunohistochemistry Study of Testis Chromogranin Immunoreactive Cells in Heroin Dependent Rats

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摘要目的:探讨大鼠睾丸嗜铬颗粒素A(CgA)免疫反应细胞在海洛因依赖期间的作用。方法:应用免疫组织化学SABC法及图像分析方法,研究大鼠海洛因依赖期间睾丸CgA免疫反应(IR)细胞形态学及功能的变化。结果:在生精小管之间的睾丸间质内可见到CgA-IR细胞,阳性产物存在于细胞胞质中;与正常组及对照组比较,海洛因依赖组大鼠睾丸CgA-IR细胞免疫染色强度明显减弱,阳性细胞数量明显减少(P<0.05);图像分析结果表明,CgA-IR细胞的平均灰度值在海洛因依赖期间呈增高趋势(P<0.05),尤以17d时最高。结论:在海洛因依赖期间,大鼠睾丸CgA-IR细胞数量减少、分泌功能减弱,与机体的调节过程有关。 Objective： To explore the possible function of Chromogranin A immunoreactive （CgA- IR） cells in rat testis during heroin dependence. Methods： Immunohistochemical SABC method and imagine analysis were used to study the morphological and functional changes of CgA-IR cells in rat tes-tis during heroin dependence. Results： The CgA-IR cells were located in the interstitial tissue between the seminiferous tubules, and the positive substances were in the cytoplasm. As compared with normal control group and saline control group, in heroin dependent group the number of CgA-IR cells （P 〈 0. 05）, and the immunohistoehemical staining intensity decreased evidently. The result of image analysis showed that the average gray degree of the CgA -IR cells increased during heroin dependence （P 〈 0. 05 ）, especially on the 17th day. Conclusions： The number and the secretion function of testis CgA-IR cells decrease during heroin dependence which might be involved in the physiological regulation of her- oin dependence.

作者郭斌梁文妹

机构地区贵阳医学院组织学与胚胎学教研室贵阳医学院山西阳泉市市人民医院

出处《贵阳医学院学报》 CAS 2008年第6期668-670,673,共4页 Journal of Guiyang Medical College

基金贵州省优秀科技人才省长基金[(2005)14号] 高层次人才基金[(2007)26号]

关键词嗜铬颗粒睾丸海洛因依赖免疫组织化学大鼠 SPRAGUE-DAWLEY chromaffin granules testis heroin dependence immunohistochemistry rats, sprague- dawley

分类号 R329.464 [医药卫生—人体解剖和组织胚胎学] R996 [医药卫生—毒理学]

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参考文献11

1苏木金,戴廷恩,张豫,苏瑾.海洛因成瘾对下丘脑神经内分泌激素系统的影响[J].中华核医学杂志,1999,19(2):111-112. 被引量：16
2潘贵书徐国强李淑芳等.海洛因成瘾大鼠模型的建立.贵阳医学院学报,1997,22:46-46.
3Abbona G, Papotti M, Viberti L, et al. Chromogranin A gene expression in non-small cell lung carcinomas[ J]. J Pathol, 1998 ( 2 ) : 151 - 156.
4Baudin E, Gigliotti A, Ducreux M,et al. Neuron-specific enolase and chromogranin A as markers of neuroendocrine turnouts[J]. Br J Cancer,1998(8) :1102 - 1107.
5Schafer MK, Nohr D, Romeo H, et al. Pan-neuronal expression of chromogranin A in rat nervous system [ J ]. Peptides, 1994 ( 2 ) :263 - 279.
6冯超,李汉忠,严维刚,罗玉凤,曹金玲.嗜铬素A和突触素在肾上腺肿瘤中的表达及其临床意义[J].中华肿瘤杂志,2005,27(8):486-488. 被引量：13
7陈丽华,张远强,苏慧慈.大鼠胰腺嗜铬颗粒素A分布的免疫组织化学研究[J].中国组织化学与细胞化学杂志,1996,5(4):411-414. 被引量：2
8Portela Gomes GM , Stidsberg M. Chromogranin A in the human gastrointestinal tract : an immunocytochemical study with region-specific antibodies [ J ]. J Histochem Cytochem, 2002(11):1487 -1492.
9Tatemoto K, Efendic S, Mutt V, et al. Pancreastatin, a novel pancreastic pPep tide that Inhibits insulin secretion [ J]. Nature, 1986(324) :476 -478.
10Winkler H, Fisher-Colbrie R. The chromogranin A and B :the first 25 years and future perspectives [ J ]. Neuroscience, 1992(3 ) :497 - 528.

二级参考文献13

1王志纲,赵金栓,赵竹林,杨毓章,张宪武,夏艳民,党仁,张捷.烫吸海洛因成瘾者血中TSH、T_3、T_4、C肽及睾酮含量变化的研究[J].中国药物依赖性通报,1994,3(4):243-246. 被引量：3
2沈敏,向平,沈保华.体内海洛因代谢产物的分析研究[J].法医学杂志,1994,10(1):27-34. 被引量：22
3沈敏，法医学杂志，1994年，10卷，27页
4王志刚，中国药物依赖性通报，1994年，3卷，243页
5姜佐宁，药物滥用，1992年，64页
6李密，药物滥用与药物依赖性，1992年，50页
7Hoffman K,Gil J,Barba J, et al.Morphometric analysis of benign and malignant adrenal pheochromocytoma.Arch Pathol Lab Med,1993,117:244-247.
8Rao F,Keiser HR,O′Connor DT. Malignant pheochromocytoma. Chromoffin granule transmitters and response to treatment.Hypertension,2000,36:1045-1052.
9Rosai J.Ackerman′s Surgical Pathology. 8th ed.New York:Mosby-Year Book Inc, 1996.1021.
10Komminoth P,Roth J,Schroder S, et al.Overlapping expression of immuno-histochemical markers and synaptophysin mRNA in pheochromocytoma and adrenocortical carcinomas,implications for the differential diagnosis of adrenal gland tumors.Lab Invest,1995,72:424-431.