摘要
通过胚胎移植验证构建的广西巴马小型猪克隆胚是否可以发育到期。利用刺入式手术胚胎移植法,将0.5～1.5日龄巴马小型猪克隆胚移植到2头巴马小型猪和2头陆川猪的输卵管壶腹部。其中2头巴马小型猪和1头陆川猪返情,另外一头陆川猪于2007年10月13日产下1头克隆雄性巴马小型猪。说明巴马小型猪克隆胚能够在受体猪体内发育到期并产仔。
The objective of this study was to examine if Guangxi Bama Mini-pig somatic cell nuclear transfer （SCNT） embryos can develop to term after embryo transfer. Bama Mini-pig cloned embryos of 0.5 - 1.5 days old were surgically transferred to the oviductal ampulla of two female Bama Mini-pigs and two female Guangxi Luchuan pigs, of which the two female mini-pigs and one Luchuan pig returned to estrns, and one female Luchuan pig gave birth of one male cloned Bama mini-piglet. It is concluded that Guangxi Bama Mini-pig SCNT embryos can develop to term after embryo transfer.
出处
《动物学杂志》
CAS
CSCD
北大核心
2008年第6期147-153,共7页
Chinese Journal of Zoology
基金
国家自然科学基金项目(No.30660127)
广西科学基金项目(桂科青0640002)
广西亚热带生物资源保护利用重点实验室-省部共建国家重点实验室培育基地开放课题(SB0701)
关键词
广西巴马小型猪
体细胞核移植
睾丸成纤维细胞
胚胎移植
Guangxi Bama Mini-pig
Somatic cell nuclear transfer
Testicle fibroblast cells
Embryo transfer
