摘要
目的:研究从海水中分离出的腐败斯瓦尼菌的致病性。方法:毒力实验组:22只二级昆白小鼠随机分为4组:实验组腹腔注射腐败斯瓦尼菌,阳性对照组注射标准金葡菌和标准大肠菌,阴性对照组注射无菌生理盐水。伤口感染组:22只SPF小鼠,腿部致伤后随机分为4组:实验组(浸泡含有细菌的人工海水)和阳性对照组(含有标准金葡菌、标准大肠菌),阴性对照组(浸泡无菌人工海水)。观察小鼠一般状况、白细胞、血培养、脏器和伤口培养及病理检查。结果:毒力实验:实验组12 h血培养阳性率28.6%;脏器阳性率42.9%。伤口感染实验:伤口感染阳性率100%,脏器培养阳性率28.6%;病理学检查伤肢肌层水肿、皮下横纹肌间弥漫大量嗜中性粒细胞。结论:自海水中分离出的腐败斯瓦尼菌在106CFU.mL-1时,具有感染能力,可致小鼠伤口、脏器感染和败血症。
Objective： To study the pathogenicity of the Shewanella putrefaciens separated from the coastal seawater.Methods： Virulence experiment groups： 22 mice were divided into four groups in random： The Shewanella putrefaciens groups were treated by intraperitioneal injection with Shewanella putrefaciens.The positive control groups were treated with standard S.aureus and E.coli were respectively in the same way,and negative control group were with stroke-physiogical saline solution.wound infection groups： 22 SPF mice were divided into four groups in random after their legs were injured： the test group（dipped in artificial seawater with the Shewanella putrefaciens）;the positive control group（with standard S.aureus and E.coli）;the negative control group（dipped in aseptic artificial seawater）.To observe the general condition,leucocyte,blood culture,viscera and wound culture of the mice,the viscera and the pathologic changes of viscera and injured extremities were examined with light microscope wound limbs were examined with pathological optical microscope.Results： Virulence experiment： among 7 mice of the experimental group,bacterial growing was found in the blood of 2 mice after injection,the positive ratio was 28.6%,the positive rate of viscera culture was 42.9%.Wound infection experiment： positive rate of the wound infection was 100%,the positive culture rate of the organ culture（of the sacriced mice） was 28.6%,pathological examination showed there were obvious dropsy in the muscular layer and widespread neutrophile granulocytes in the cross striated muscle of the injured extremity.Conclusion： The Shewanella putrefaciens isolated from the brine has transmissibility,which can cause wound（and riscera） infection and septicaemia when the concentration reached 106CFU·mL^-1.
出处
《军医进修学院学报》
CAS
北大核心
2008年第6期487-488,共2页
Academic Journal of Pla Postgraduate Medical School
基金
全军医药卫生指令性课题(2004)
关键词
毒性试验
海水
损伤和创伤
腐败斯瓦尼菌
伤口感染
toxicity tests
seawater
wounded and injuries
Shewanella putrefaciens
wound infection
