摘要
为建立鉴定大豆杂种的方法,采用亲本间有多态性的3对SSR引物,对148个耐盐与盐敏感大豆品种正反交F1植株进行分子鉴定,结果表明,有81.8%的F1为真杂种,且3对多态性SSR引物检测结果一致;在亲本基因型纯合的情况下,采用1对在亲本间有多态性的SSR引物即可对F1真伪进行准确判断;亲本间分子量差异大的SSR位点可用高浓度琼脂糖电泳进行快速鉴定。利用该方法对2007年参加国家大豆区试的大豆杂交种品系H02-286的50粒种子进行纯度鉴定,进一步验证了SSR标记检测杂种真伪的可行性。
In order to test the verity of hybrids derived from the cross of salt tolerant and salt sensitive cultivars, three polymorphic SSR markers between soybean parents were selected to evaluate 148 hybrids. The purity ratio of soybean hybrids is 81.8% , and the results identified by different SSR markers were identical. This study indicated, if the parents were pure lines, that a single appropriate SSR marker can be used to test seed purity of the hybrids. The SSR loci with great fragment length different between the parents should be selected in order to identify the genotypes by agarose gel. In order to estimate the effectiveness of this method, fifty seeds of a hybrid line named H02-286 were used as materials for further research, and the result showed the feasibility of this method.
出处
《植物遗传资源学报》
CAS
CSCD
2008年第4期443-447,共5页
Journal of Plant Genetic Resources
基金
国家自然科学基金项目(30490250
30671310)
国家高科技研究发展计划("863"计划)(2006AA101104
2006AA10Z1B3)
