摘要
研究了性逆转金鳟雄鱼（XX，伪雄鱼）精子在不同pH的人工精浆（ artificial seminal plasma,ASP）溶液中的活动情况。结果显示，随着pH的提高精子活力不断增强。伪雄鱼精子在不同ASP—pHs中培养1h后，pH9．5～10．5培养组精子活力超过正常雄鱼精子（XY）活力水平，且正常雄鱼精子与pH8．5～9．5培养组的精子活力不存在显著性差异（P〉0．05）。将金鳟伪雄鱼精液培育在pH8．5～10．5的ASP中，0～180min内，pH10．5中的精子寿命和激活比例都最佳，180min之后的精子寿命超过80s，激活比例在90％以上，显著好于其他pH组（P〈0．05）。在低温储藏12h后，正常雄鱼精子在ASP—pH10．5溶液中寿命时间最长，为（284．05±78．37）S，其次是同等稀释条件下的伪雄鱼精子寿命，其时间为（203．89±43．68）s，正常雄鱼精液原浆精子寿命最短，为（122．19±37．61）s。直接用金鳟伪雄鱼精液受精，发眼率和仔鱼上浮率仅为12．50％和8．77％，而通过pH10．5的ASP培养后，其发眼率和孵化率分别提高到了52％和49．95％。因此，在高pH的ASP中培养金鳟伪雄鱼精子能提高其活力和延长寿命。通过这种处理也能够显著的提高金鳟全雌和全雌三倍不育后代的育苗生产效率。
The sperm motility of sex-reversed male golden rainbow trout （XX） in different pHs of artificial seminal plasma was studied. The result showed that the motility of sperm was uninterruptedly enhanced with the raise of pH. After being incubated in different pHs of artificial seminal plasma for 1 h, the sperm motility of sex-reversed male incubated in pH 9.5 - 10.5 of artificial seminal plasma exceeded that of normal male （XY）, and there wasn＇ t significant difference between the sperm motility of normal male and that of sex-reversed male incubated in pH 8.5 - 9.5of artificial seminal plasma （P 〉 0.05 ）. To incubate sperm of sex-reversed golden rainbow trout male in ASP-pHs （8.5 - 10.5）, for 0 - 180 min, the sperm life and activation ratio of sperm in pH 10.5 of ASP were best, after 180 min, the sperm life exceeded 80 s, and the activation ratio of sperm was above 90%, which were significantly better than that incubated in other pH series （P 〈 0.05 ）. After being stored 12 h at subambient temperature, the sperm life of normal male incubated in pH 10.5 of ASP was longest, which was （284.05 ±78.37） s; next, the sperm longevity of sex-reversed male was （203.89 ±43.68） s, and the sperm life of normal male was shortest that was （ 122.19 ±37.61 ） s. The eyed ratio and flying up ratio of fertilizing by sperm of sex-reversed male golden rainbow trout were only 12.50% and 8.77%, respectively, but the eyed ratio and flying up ratio of fertilizing by sperm of sexreversed male golden rainbow trout incubated in pH 10.5 of ASP were up to 52% and 49.95%. Thus, the incubation of the sex-reversed male golden rainbow trout sperm in high-pH artificial seminal plasma improved the motility and life of spermatozoa. By this treatment, it is possible to markedly increase production efficiency of raising seedling of all-female golden rainbow trout and all-female triploid sterile progenies.
出处
《上海水产大学学报》
CSCD
北大核心
2008年第6期647-654,共8页
Journal of Shanghai Fisheries University
基金
黑水研基本科研专项(2007HSYZX-YY-21)
黑龙江省科技攻关项目(GC03B511GA06B203-4)
科技部农转资金项目(2007GB23260395)
国家“十一五”支撑计划(2006BAD03B08)
公益性行业科研专项淡水重要经济鱼类产业化研究与示范(200803050)
关键词
金鳟
人工精浆
性逆转雄鱼
精子活力
golden rainbow trout （ Oncorhynchus mykiss ）
artificial seminal plasma （ ASP ）
sex reversal milter
sperm motility
