摘要
目的:本研究探讨经Gln处理后对内毒素(LPS)刺激健康人外周血单个核细胞(PBMC)细胞因子表达的影响。方法:取健康志愿者新鲜外周血,用密度梯度离心法提取出单个核细胞,以LPS刺激单个核细胞后,分别使用不同浓度(1、3、6、8、10和15mmol/L)Gln处理2h后,用ELISA法检测细胞上清液中的细胞因子(TNF-α、IL-1β、IL-4、IL-6、IL-8、IL-10)水平,并利用SuperArray基因芯片技术检测细胞因子mRNA。结果:LPS刺激PBMC后,细胞因子大量表达,用Gln处理能显著减少细胞因子的转录和转录后蛋白的表达(P<0.05),且这一调控作用与Gln剂量呈正相关。结论:Gln能下调LPS刺激后PBMC细胞因子的表达。
Objective ： The effect of glutamine on the cytokine expression in peripheral blood mononuclear cells stimulated by lipopolysaccharide was investigated. Methods： We employed the density gradient centrifugation to extract PBMC from fresh peripheral blood of healthy volunteers. The cytokines（TNF-ot, IL-1 α, IL-4, IL-6, IL-8, IL-10）were observed after the PBMC was treated by LPS. Suspension of PBMC was treated with the concentrations of 1, 3, 6, 8, 10, and 15 mmol/L Gin for 4, 12, and 24 h respectively. The expression of the cytokines was detected in the supernatant of PBMC with Enzyme-Linked Im- munosorbnent Assay, and the mRNA of these cytokines was detected in cells with Gene Array Protocol. Results： After LPS stimulation, an excessive expression of the cytokines was observed. These cyto- kines were reduced significantly by glutamine treatment at the transcriptional level and the posttranscrip- tional level （P 〈 O. 05 ）. And the attenuation was directly correlated with the dose of Gin. Conclusion： Glutamine can down-regulate the cytokine expression of PBMC stimulated by LPS.
出处
《肠外与肠内营养》
CAS
2008年第6期323-325,328,共4页
Parenteral & Enteral Nutrition
基金
国家自然科学基金资助项目(30500404)
