摘要
目的：通过对道地产区山茱萸果实有效成分变异、有效成分问及有效成分与外形、营养指标之间的相关研究，为山茱萸基于有效成分的良种选择提供理论基础。方法：采集山茱萸道地产区浙江省淳安县、临安市实生单株完熟果实，运用HPLC测定单株果实的马钱素、熊果酸、齐墩果酸含量，同时测定山茱萸果实的纵径／横径、可溶性固形物、出皮率等性状。结果：①道地产区内熊果酸、齐墩果酸、马钱素变异幅度分别为0．1010％-0．4786％，0．0149％～0．1274％，0．59％～2．30％，RSD分别为34．33％，40．48％，28．50％；②有效成分间及有效成分与外形、营养指标之间的相关关系如下：熊果酸、齐墩果酸相关极显著，相关系数为0．9796；熊果酸、齐墩果酸与可溶性固形物成极显著负相关，相关系数分别为-0．5544，-0．5118；马钱素与可溶性固形物成显著相关，相关系数为0．2412；熊果酸、齐墩果酸、马钱素与出皮率成显著负相关，相关系数分别-0．2507，-0．2443，-0．2406；3种有效成分与纵径／横径相关不明显。结论：山茱萸单株间有效成分变异明显，选种潜力巨大；利用有效成分间及有效成分与外形、营养指标之间的相关关系，进行以有效成分为指标的选育种时，应以单株选种为主，可将熊果酸与齐墩果酸合并作为1个指标，并可用可溶性固形物做粗步筛选。
Objective： In order to provide a theoretical foundation for the medically effective ingredient-based selection of elite cuhivars in Comus officinalis, a study has been conducted on the variation in medicinal effective ingredients of the fruit from the genu- ine producing areas, the correlation among effective medicinal ingredients and the correlation between effective ingredients and fruit shape as well as nutritional indexes. Method： The completely mature fruit was collected from the genuine producing areas Chunan county and Lin＇an city of Zhejiang province. The contents of colchicine, ursolic acid and oleanolic acid were determined by HPLC, and vertical diameter/transversal diameter of the fruits, soluble solid matter and percentage of fresh flesh to the fruit were also measured. Result： ① Ursolic acid, oleanolic acid, and colchicine in fruits ranged from 0. 101 0% to 0.478 6%, 0. 014 9% to 0. 127 4% and 0.59% to 2.30%, respectively, and their RSD were 34.33%, 40.48% and 28.50%, respectively. ② The correlation between effective ingredients and that between effective ingredients and fruit shape as well as nutritional indexes were as follows： the content of ursolic acid was significantly correlated with that of oleanolic acid with a correlation coefficient of 0.979 6 ; both ursolic acid and oleanolic acid were in significantly negative correlation with soluble solid matter with a correlation coefficient of - 0.554 4 and - 0.511 8, respectively; colchicine was significantly associated with soluble solid matter with a correlation coefficient of 0.241 2; colchicine, ursolic acid and oleanolic acid were in significantly negative correlation with the percentage of fresh flesh with a correlation coefficient of - 0. 250 7, - 0. 244 3 and - 0. 240 6, respectively ; three effective ingredients showed no correlation with the ratio of vertical diameter to transversal diameter of the fruit. Conclusion： There is a significant difference in effective ingredients among individual trees, which means that there is a big potential for selection of cuhivars. Individual tree-based selection should be mainly adopted when effective ingredients are used as a main index in selection on the basis of the correlation among effective ingredients and that between effective ingredients and fruit shape as well as nutritional indexes, while ursolic acid could be combined with oleanolic acid to be used as an index and a preliminary screen could be conducted using soluble solid matter.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第23期2751-2755,共5页
China Journal of Chinese Materia Medica
基金
浙江省重中之重学科浙江林学院森林培育学科开放基金(200511)
浙江省教育厅项目(2451001066)
关键词
山茱萸
有效成分
变异
性状相关
Comus officinalis
effective ingredient
variation
correlation between traits
