摘要
目的:研究野菊花总黄酮(TFC)对佐剂性关节炎(AA)大鼠滑膜细胞的凋亡诱导作用,探讨TFC治疗AA的可能机制。方法:SD大鼠随机分成6组:正常组、模型组、TFC高、中、低剂量组和阳性药雷公藤多苷(TPT)组;SD大鼠右后足跖皮内注射弗氏完全佐剂复制AA模型;MTT法检测滑膜细胞的增殖;原位末端标记检测(TUNEL)法观察滑膜组织中滑膜细胞的凋亡改变;流式细胞仪检测体外培养滑膜细胞的凋亡变化。结果:AA大鼠滑膜细胞出现凋亡抑制,增殖过度的类瘤样细胞特征,TFC可剂量依赖性地降低AA大鼠滑膜细胞的炎性增殖;TFC(168,336 mg.kg-1)治疗组大鼠滑膜细胞的凋亡率较模型组显著升高。结论:TFC可抑制AA大鼠滑膜细胞的过度增殖,诱导滑膜细胞凋亡,这可能是其治疗AA的机制之一。
Objective: To investigate the effect on the extract of total flavonoids of Chrysanthemum indicum (TFC) on adjuvant arthritis synovial cells. Method: SD rats were divided randomly into six groups including normal, model, TFC (84, 168,336 mg · kg^-1 ) and control drug Tripterygium glycosides (30 mg· kg^-1 ) groups. Adjuvant arthritis rat model was induced by a single intradermal injection of 0. 1 mL of the complete Freund's adjuvant into the right hind feet pads of the SD rats. The proliferation of synoviocyte was measured by MTT; The apoptosis rates of synovial cells were evaluated using TUNEL and FCM analysis. Result: TFC resulted in a dose-dependent way in inhibiting the proliferation of synovial and inducing the apoptosis of synovium and synoviocytes in vivo. Conclusion: TFC can inhibit proliferation and induce apoptosis in synovial cells, and exert therapeutical effect on rheumatoid arthritis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第23期2838-2841,共4页
China Journal of Chinese Materia Medica
基金
国家科技部重大基础研究项目(2002CCCC02900)
安徽医科大学校科研基金项目(2006kj20)
关键词
野菊花总黄酮
佐剂性关节炎
细胞凋亡
原位末端标记检测法
流式细胞术
total flavonoids of Chrysanthemum indicum (TFC)
adjuvant arthritis
apoptosis
TUNEL ( TdT-nediated dUTP nick end labeling)
flow cytometer(FCM)