摘要
目的研究电针加灸对急性脑缺血再灌注大鼠血脑屏障的保护作用与血清中S100β蛋白水平的相关性。方法采用大脑中动脉线栓法制备缺血再灌注模型,造模成功后30min,电针加灸组电针"百会"、"水沟"穴,艾灸"足三里"穴。测定各组治疗前后6h、12h、24h、48h后大鼠血清中S100β蛋白含量,观察各组治疗前后各时相大鼠血脑屏障超微结构的变化。结果(1)H-600透射电镜显示,电针加灸组大鼠血脑屏障破坏程度较模型组轻微。(2)ELISA结果显示:电针加灸组中S100β蛋白的含量明显低于模型组(P<0.05)。结论电针加灸能降低急性脑缺血再灌注大鼠外周血清中的S100β含量,对血脑屏障的损伤有一定的保护作用。
Objective： To investigate the protection BBB by using electro- acupuncture and moxibustion after acute cerebral ischemia reperfusion in rats and the dynamic changes of S100βprotein level in serum. Method ： Use middle cerebral artery occlusion / reperfusion （MCAO / R） rat model. 30 minutes later the ＂Baihui＂ and ＂Shuigou＂ points were stimulated by electro - acupuncture and ＂Zusanli＂ point was stimulated by moxbustion. To investigate and evaluate the S100β protein level in blood serum after 6h, 12 h, 24 h, 48h of the treatment. Then observe the uhramicrostmcture change of the BBB of each phase before and after the treatment. Results ： （ 1 ） H - 600 transmission electron microscope shows that damage of the BBB system in electro - acupuncture and moxibustion phase are lighter than that in model phase. （2） ELTSA results show that S100β protein level in electro - acupuncture and moxibustion group is lower than that in model group （ P 〈 0.05）. Conclusions ： Electro - acupuncture and moxibustion can protect the BBB system and cut down the S100β protein level in blood serum, keeping the normal physiologic function.
出处
《湖北中医学院学报》
2008年第4期8-11,共4页
Journal of Hubei College of Traditional Chinese Medicine
基金
湖北中医学院资助课题
[中医院字2007
244号]
关键词
脑缺血再灌注
血脑屏障
S100Β
Cerebral ischemia- reperfusion
Blood brain barrier
S100β
