摘要
目的:建立六味地黄颗粒(熟地黄,山茱萸,牡丹皮,山药,等)中马钱苷和丹皮酚的HPLC含量测定方法。方法:采用DIONEX Acclaim色谱柱,马钱苷以乙腈-水(15:85)为流动相,检测波长为236nm;丹皮酚以甲醇-水(70:30)为流动相,检测波长为274nm。结果:马钱苷和丹皮酚的线性范围分别为2.34～234μg/mL(r=0.99999)、7.07～141.40μg/mL(r=0.9996),平均回收率分别为100.06(RSD为3.10,n=9)、95.75(RSD为0.65,n=6)。结论:该方法操作简便准确,重现性好,能有效控制六味地黄颗粒的质量。
AIM： To establish a method for determing loganin and paeonol in Liuwei Dihuang Granules（ Radix Rehmannial praeparata , Fructus Corni , Cortex Moutan , Rhizoma Dioscoreae , etc. ）. METHODS ： Loganin was determined using acetonitrile-water （15： 85） as the mobile phase with UV detection at 236 nm, and paeonol was determined using methanol-water （70： 30） as the mobile phase with UV detection at 274 nm by HPLC with Diamonsil C18 （150 mm ×4.6 mm, 3 μm） column. RESULTS： The linear ranges of loganin and paeonol were in the ranges of 2.34 - 234 μg/mL（ r = 0.999 99） ,7.07 - 141.40 μg/mL（ r = 0. 999 6 ）, respectively. The average recoveries of toganin and paeonol were 100.06% ,95.75% with RSD of 3. 10% ,0.65%, respectively. CONCLUSION： This method is simple, accurate, reproducible and can be used for determining loganin and paeonol in Liuwei Dihuang Granules.
出处
《中成药》
CAS
CSCD
北大核心
2008年第12期1791-1794,共4页
Chinese Traditional Patent Medicine
