摘要
建立了同时测定鸡肉中莫能菌素和盐霉素残留检测方法。在国内外已有研究的基础上，对样品前处理和仪器条件进行了改进，采用乙腈提取鸡肉中莫能菌素和盐霉素残留，石墨化碳黑SPE小柱对提取液进行净化，然后用Waters ACQUITY UPLC^TM TQD超高效液相色谱-串联质谱仪测定。结果表明，莫能菌素和盐霉素的检测限为0.2μg／kg，最低定量限为0.5μg／kg。选取0.5、1.0、2.0μg／kg三个浓度进行空白添加回收率实验，回收率为71.9％-95.1％，批内变异系数和批间变异系数均小于15％，适合大量鸡肉样品中莫能菌素和盐霉素的残留检测。
A method of the simultaneous analysis of monensin and salinomycin residues in chicken muscles was developed with Ultra Performance Liquid Chromatography - Tandem Mass Spectrometry. The residues from chicken muscles were extracted with acetonitrile, and the extracting solutions were purified with supelclean ENVI-Carb SPE cartridges. The monensin and salinomycin residues were separated on a Waters ACQUITY UPLC^TM BEH C18 column, and detected by MS/MS with positive Electrospray Ionization mode. The limit of detection （LOD） for the two drugs was 0.2μg/kg, and the limit of quantification （LOQ） was 0.5μg/kg. At three spiking levels of 0.5, 1.0,2.0 μg/kg, average recoveries were between 71.9% and 95. 1%, Intraassay coefficient of variation and interassay coefficient of variation were below 15%. The experiment results showed that this method was simple, rapid and applicable to simultaneously detect residues of monensin and salinomycin in chicken muscles.
出处
《中国兽药杂志》
2008年第12期23-26,共4页
Chinese Journal of Veterinary Drug
基金
"十一五"国家科技支撑计划"新兽药筛选与安全评价技术研究"(2006BAD31B09)
